OVERVIEW | |
Organism | Human (Homo sapiens), 11 years old white female (Ref: ATCC) |
Source/Tissue of origin | Bone (Ref: ATCC, culturecollections) |
Year of isolation | 1973 (Ref: Cabri-ACC 243) |
Morphology | Epithelial (Ref: ATCC) |
Cell type | Epithelial-like (Ref: culturecollections) |
Growth mode | Adherent (atcc, culturecollections) |
Doubling time | 37 h (Rodan et al., 1987) |
Life span | Infinite, continuous culture |
Karyotype | Hypotriploid with 56 modal chromosome number (Ref: ATCC) |
Transformed | Form colonies in semisolid medium (Ref: ATCC) |
Tumorigenic | No, not tumorigenic in immunosuppressed mice (Rodan et al., 1987) |
Drug resistance | ? |
Gene Expression | |
Positive for | TGFβ (type 1 and type 2) (Ref: ATCC) Parathyroid hormone receptor (Rodan et al., 1987) 1,25(OH)2D3 receptors (Rodan et al., 1987) Osteonectin (Rodan et al., 1987) |
Altered gene | Express truncated form of pRB (inactive form, cytoplasmic) (Hinds et al., 1992) Do not express p53 (Marcellus et al., 1996) |
Enzymes | Positive for alkaline phosphatase (Rodan et al., 1987) |
Products | ? |
Applications | 3D cell culture (Ref: ATCC) Suitable as a transfection host (Ref: ATCC) |
Remarks | |
Keywords | Human primary osteogenic sarcoma |
Related cell lines | |
Culture maintenance | |
Biosafety Level | BSL 1 (Ref: ATCC) (It may differ in your country, follow the guidelines of your institute/country) |
Culture medium* | 1. McCoy’s 5a + 2mM L-Glutamine + 15% FBS 2. DMEM + 2mM L-Glutamine + 10% FBS |
Culture conditions | Humidified incubator, 95% air + 5% CO2, 37°C |
Subculturing procedure | Trypsin-EDTA |
Culture storage | |
Cryo-medium | Complete growth medium + 5% (v/v) (Ref: ATCC) |
Storage conditions | Vapor phase of liquid nitrogen (Ref: ATCC) |
* Several culture media can be used to maintain this cell line in culture. Use the culture medium recommended by the suppliers/source from where you have received this cell line.
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