| OVERVIEW | |
| Organism | Human (Homo sapiens), 11 years old white female (Ref: ATCC) |
| Source/Tissue of origin | Bone (Ref: ATCC, culturecollections) |
| Year of isolation | 1973 (Ref: Cabri-ACC 243) |
| Morphology | Epithelial (Ref: ATCC) |
| Cell type | Epithelial-like (Ref: culturecollections) |
| Growth mode | Adherent (atcc, culturecollections) |
| Doubling time | 37 h (Rodan et al., 1987) |
| Life span | Infinite, continuous culture |
| Karyotype | Hypotriploid with 56 modal chromosome number (Ref: ATCC) |
| Transformed | Form colonies in semisolid medium (Ref: ATCC) |
| Tumorigenic | No, not tumorigenic in immunosuppressed mice (Rodan et al., 1987) |
| Drug resistance | ? |
| Gene Expression | |
| Positive for | TGFβ (type 1 and type 2) (Ref: ATCC) Parathyroid hormone receptor (Rodan et al., 1987) 1,25(OH)2D3 receptors (Rodan et al., 1987) Osteonectin (Rodan et al., 1987) |
| Altered gene | Express truncated form of pRB (inactive form, cytoplasmic) (Hinds et al., 1992) Do not express p53 (Marcellus et al., 1996) |
| Enzymes | Positive for alkaline phosphatase (Rodan et al., 1987) |
| Products | ? |
| Applications | 3D cell culture (Ref: ATCC) Suitable as a transfection host (Ref: ATCC) |
| Remarks | |
| Keywords | Human primary osteogenic sarcoma |
| Related cell lines | |
| Culture maintenance | |
| Biosafety Level | BSL 1 (Ref: ATCC) (It may differ in your country, follow the guidelines of your institute/country) |
| Culture medium* | 1. McCoy’s 5a + 2mM L-Glutamine + 15% FBS 2. DMEM + 2mM L-Glutamine + 10% FBS |
| Culture conditions | Humidified incubator, 95% air + 5% CO2, 37°C |
| Subculturing procedure | Trypsin-EDTA |
| Culture storage | |
| Cryo-medium | Complete growth medium + 5% (v/v) (Ref: ATCC) |
| Storage conditions | Vapor phase of liquid nitrogen (Ref: ATCC) |
* Several culture media can be used to maintain this cell line in culture. Use the culture medium recommended by the suppliers/source from where you have received this cell line.
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