Escherichia coli DH5α

  • One of the most common laboratory Escherichia coli strains used for molecular cloning
  • Derived from Escherichia coli K-12
  • High transformation efficiency
  • Appropriate host for α-complementation and blue-white selection
  • Mutations in recA1 and endA1 in this strain provide better insert stability during cloning
  • Can also be used for the assembly of DNA fragments (Kostylev, et al., 2015)
  • DH5α is a trademark of Invitrogen Corp. (Sigma-Aldrich)


  • Kostylev, et al., 2015. Cloning Should Be Simple: Escherichia coli DH5α-Mediated Assembly of Multiple DNA Fragments with Short End Homologies. PLoS One. 10(9), e0137466. PMID-26348330


  • Jung et al., 2010. Restoration of growth phenotypes of Escherichia coli DH5alpha in minimal media through reversal of a point mutation in purB. Appl Environ Microbiol. 76(18), 6307-9. PMID-20675450; Full-Text Links: ASM (Download PDF), PMC 
  • Anton & Raleigh, 2016. Complete Genome Sequence of NEB 5-alpha, a Derivative of Escherichia coli K-12 DH5α. Genome Announc. 4(6), e01245-16. PMID-27834703; Full-Text Links: ASM (Download PDF), PMC
  • Song et al., 2015. Determination of single nucleotide variants in Escherichia coli DH5α by using short-read sequencing.  FEMS Microbiol Lett. 362(11), fnv073. PMID-25934703; Full-Text Links: Oxford University Press (Download PDF)

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