Category: Lab Notes: Biotechnology
trp Promoter
The trp promoter is a naturally occurring promoter derived from the tryptophan (trp) operon of Escherichia coli, which controls genes…
T7 Lysozyme
T7 lysozyme is a bacteriophage-encoded protein that serves a dual role: it functions both as a lytic enzyme during the…
BL21 Vs BL21(DE3) Vs BL21(DE3)pLysS
Criteria BL21 BL21(DE3) BL21(DE3)pLysS Remarks Genetic Background Wild-type E. coli B strain derivative BL21 containing the λDE3 lysogen, which harbors…
BL21(DE3)pLysS vs. BL21(DE3) CodonPlus
Criteria BL21(DE3)pLysS BL21(DE3) CodonPlus Remarks Genetic Background Derivative of E. coli BL21(DE3) containing the pLysS plasmid Derivative of BL21(DE3) engineered…
T7 RNA Polymerase
T7 RNA polymerase is a highly specific and efficient RNA polymerase derived from bacteriophage T7, widely used in molecular biology…
In Vitro T7 Transcription System
The in vitro T7 transcription system is a widely used tool in molecular biology that harnesses the powerful and specific…
pLysS System Vs pLysE System
Criteria pLysS System pLysE System Remarks Encoded Protein T7 Lysozyme (low expression level) T7 Lysozyme (high expression level) Both express…
SP6 RNA Polymerase
SP6 RNA polymerase is a DNA-dependent RNA polymerase derived from Salmonella bacteriophage SP6, commonly used for in vitro transcription of…
T3 RNA Polymerase
T3 RNA polymerase is a DNA-dependent RNA polymerase derived from bacteriophage T3, closely related to T7 RNA polymerase, but with…
T3 Promoter
The T3 promoter is a highly specific DNA sequence recognized exclusively by T3 RNA polymerase, originating from bacteriophage T3. It…
SP6 Promoter
The SP6 promoter is a short, well-characterized DNA sequence recognized specifically by SP6 RNA polymerase, derived from Salmonella bacteriophage SP6. …
Ptac Promoter
The Ptac promoter is a synthetic hybrid promoter created by combining elements of the trp and lac promoters, designed to…
araBAD Promoter
The araBAD promoter is a tightly regulated and inducible promoter derived from the arabinose operon of Escherichia coli, which controls…
T7 Promoter
The T7 promoter is a bacteriophage-derived promoter that is widely used for high-level expression of recombinant proteins in Escherichia coli…
Escherichia coli SHuffle
Escherichia coli SHuffle is a specially engineered strain developed to enhance the correct folding of disulfide bond-containing proteins in the…