Category: Lab Notes: Cell Biology
Heterokaryon
A heterokaryon is a type of cell that contains two or more genetically distinct nuclei sharing a common cytoplasm. This…
Hayflick Limit
The Hayflick limit is a fundamental concept in cellular biology that describes the finite number of times a normal somatic…
Immortalized Cell Line
Immortalized cell lines are populations of cells that have acquired the ability to proliferate indefinitely under specific culture conditions, making…
Mesoscale Organization in Biology
Mesoscale organization in biology refers to the structural and functional arrangements that occur at an intermediate scale—between the molecular level…
Skin Fibroblast
Skin fibroblasts are the principal connective tissue cells of the dermis, the supportive middle layer of the skin located beneath…
The Origin of Cell Culture: Ross Harrison’s Pioneering 1907 Experiment
Ross Granville Harrison’s 1907 experiment marks a foundational moment in the history of modern cell biology. He was the first…
Dimethyl Sulfoxide (DMSO) in Epigenetic Regulation
Dimethyl sulfoxide (DMSO), beyond its well-known applications as a solvent and cryoprotectant, has significant effects on the epigenetic landscape of…
Dimethyl Sulfoxide (DMSO) in Stem Cell Research
Dimethyl sulfoxide (DMSO) is a widely used chemical compound in stem cell research, valued primarily for its ability to preserve…
Calcium Phosphate Transfection
Calcium phosphate transfection is a widely used and cost-effective method for introducing plasmid DNA into eukaryotic cells. It is particularly…
Evolutionary Insights from the Nucleolar Proteome
The nucleolus, once regarded merely as a static structure dedicated to ribosome production, is now recognized as a dynamic and…
Transfection: Timeline of Key Events
Transfection, the process of introducing foreign nucleic acids into eukaryotic cells, has become a cornerstone of modern molecular biology, biotechnology,…
Large-Scale Transient Transfection of Mammalian Suspension Cells for Rapid Protein Production
Large-scale transient transfection of mammalian cells in suspension is a widely used method for the rapid production of recombinant proteins,…
Mitochondrial Protein Synthesis Vs Cytoplasmic Protein Synthesis (Animal Cell)
Criteria Mitochondrial Protein Synthesis Cytoplasmic Protein Synthesis (Animal Cells) Remarks Location Occurs in the mitochondrial matrix Occurs in the cytoplasm…
Mitochondrial Ribosome Vs Cytoplasmic Ribosome (Animal Cell)
Criteria Mitochondrial Ribosome (Mitoribosome) Cytoplasmic Ribosome (Animal Cell) Remarks Location Located within the mitochondrial matrix Found in the cytoplasm and…
Impact of Doxycycline on Cell Proliferation and Metabolism
Doxycycline is widely used in molecular biology and biomedical research, particularly as a regulator in inducible gene expression systems such…