Category: Lab Notes: Microbiology
Translocation-deficient cagE Knockout Strain of Helicobacter pylori
A translocation-deficient cagE knockout strain of Helicobacter pylori is a genetically modified variant in which the cagE gene—an essential component…
CagA Delivery to Host cell: Transfection Vs by Infection
Criteria Transfection-Based Delivery of CagA Infection with CagA-positive Helicobacter pylori Remarks Method Overview Introduction of plasmid DNA encoding cagA directly…
CagA-Induced Changes in MKN-28 Cells
The MKN-28 cell line, derived from moderately differentiated human gastric carcinoma, serves as a valuable model for studying Helicobacter pylori–host…
MKN-28 Cell Line in Helicobacter pylori Research
The MKN-28 cell line, derived from a human gastric carcinoma, is a moderately differentiated gastric epithelial cell line that has…
MKN-45 Cell Line in Helicobacter pylori Research
The MKN-45 cell line, derived from a poorly differentiated human gastric carcinoma, is widely used in Helicobacter pylori research due…
Model Systems for Studying Helicobacter pylori
Understanding Helicobacter pylori infection and pathogenesis has required a diverse array of experimental models, ranging from simple cell cultures to…
Caco-2 Cell Line in Helicobacter pylori Research
The Caco-2 cell line, derived from human colorectal adenocarcinoma, has been widely used in gastrointestinal research and has made valuable…
NCI-N87 Cell Line in Helicobacter pylori Research
The NCI-N87 cell line, derived from a human gastric carcinoma, has emerged as a valuable model in Helicobacter pylori research,…
MDCK Cell Line in Helicobacter pylori Research
The MDCK cell line (Madin-Darby Canine Kidney) has significantly contributed to research on Helicobacter pylori pathogenesis, particularly in understanding epithelial…
AGS Cell Line in Helicobacter pylori Research
The AGS cell line, derived from a human gastric adenocarcinoma, has played a foundational role in advancing our understanding of…
Plasmid: Geometry and Conformation
Plasmid geometry and conformation represent fundamental aspects of plasmid structure that influence their biological function and behavior in both natural…
Lactic Acid Bacteria
Lactic acid bacteria (LAB) represent a diverse group of gram-positive, non-sporing microorganisms that have been fundamental to food fermentation throughout…
Milk-associated Microorganisms
Milk-associated microorganisms represent a complex ecosystem that plays a crucial role in both the quality and safety of dairy products….
Escherichia coli Pathogenic Strains
Pathogenic strains of Escherichia coli represent distinct variants that have acquired specific virulence factors through horizontal gene transfer and evolutionary…