Category: Lab Notes: Molecular Biology
Molecular Biology of Disease
Molecular biology of disease is the study of how genetic, biochemical, and cellular processes contribute to the onset, progression, and…
Glycosidic Bond
A glycosidic bond is a crucial chemical linkage that joins carbohydrate molecules together, forming the basis for complex carbohydrate structures….
HA-Tag
The HA-tag is a widely used epitope tag derived from the human influenza hemagglutinin protein. It consists of nine amino…
Myc-Tag
The Myc-tag is a widely used polypeptide protein tag derived from the c-myc gene product. It consists of the amino…
Strep-Tag
The Strep-tag is a versatile peptide tag used in protein purification and detection, developed as an alternative to other common…
His-Tag
The His-tag (polyhistidine tag) is one of the most widely used affinity tags in molecular biology and protein purification. It…
FLAG-Tag
The FLAG tag is a widely used peptide tag in molecular biology and biochemistry, consisting of the eight amino acid…
Fusion Tag
A fusion tag is a peptide or protein sequence that is genetically engineered to be expressed alongside a protein of…
Double-Stranded RNA
Double-stranded RNA (dsRNA) is a molecular structure in which two complementary RNA strands are bound together by Watson–Crick base pairing,…
Adenosine-to-Inosine (A-to-I) RNA Editing
Adenosine-to-inosine (A-to-I) RNA editing is one of the most widespread and well-studied forms of post-transcriptional RNA modification in metazoans. It…
Transcription Start Site
A transcription start site (TSS) is the exact nucleotide position in the genome where RNA polymerase begins transcribing DNA into…
Circular RNA Vector Expression Technology
Circular RNA (circRNA) vector expression technology refers to a set of molecular tools and strategies designed to artificially synthesize and…
Circular RNA
Circular RNAs (circRNAs) are a class of endogenous non-coding RNAs characterized by their covalently closed-loop structure, which distinguishes them from…
Oligonucleotide Synthesis
Oligonucleotide synthesis is the chemical process of creating short strands of nucleic acids—typically DNA or RNA—with a defined sequence of…
Oligonucleotide
Oligonucleotides are short sequences of nucleotides—typically composed of DNA or RNA—that are synthetically produced and designed to have specific sequences….