Category: Lab Notes: Molecular Biology
Single-Stranded DNA (ssDNA)
Single-stranded DNA (ssDNA) is a DNA molecule that consists of only one of the two complementary strands that form the…
Recombinant Protein Technology: Timeline of Key Events
Recombinant protein technology represents one of the most transformative achievements in modern biotechnology, enabling the precise production of proteins through…
Protein
Proteins are complex macromolecules that serve as fundamental components of all living organisms, playing crucial roles in virtually every biological…
Topic: Gene
Gene Prokaryotic Gene Vs Eukaryotic Gene Intron-less Genes in Eukaryotes Gene Expression Analysis Gene Expression Analysis by NanoString Technology
Gene
A gene is a fundamental unit of heredity consisting of a specific sequence of DNA nucleotides that encodes functional products…
Regulation of Inducible Gene Expression Using Doxycycline in Tet-On and Tet-Off Systems
Doxycycline plays a central role in the regulation of gene expression in the Tet-On and Tet-Off systems, which are widely…
Gibson Assembly
Gibson Assembly is an efficient molecular cloning method that allows seamless assembly of multiple DNA fragments in a single reaction….
Molecular Cloning
Molecular cloning is a fundamental technique in molecular biology that enables the creation of identical DNA copies and their propagation…
Recombinant DNA Technology
Recombinant DNA technology is a cornerstone of modern biotechnology, enabling the manipulation and combination of DNA from different sources to…
Ribonuclease A (RNase A) Vs Ribonuclease T1 (RNase T1)
Criteria RNase A RNase T1 Remarks Full Name Ribonuclease A Ribonuclease T1 Both are ribonucleases but differ in sequence specificity…
Analyzing Protein Homodimerization via Differential Epitope Tagging and Co-Immunoprecipitation
Protein homodimerization—the interaction of two identical protein subunits—is a fundamental mechanism in regulating protein function, stability, and signaling. A widely…
trp Promoter
The trp promoter is a naturally occurring promoter derived from the tryptophan (trp) operon of Escherichia coli, which controls genes…
T7 Lysozyme
T7 lysozyme is a bacteriophage-encoded protein that serves a dual role: it functions both as a lytic enzyme during the…
BL21 Vs BL21(DE3) Vs BL21(DE3)pLysS
Criteria BL21 BL21(DE3) BL21(DE3)pLysS Remarks Genetic Background Wild-type E. coli B strain derivative BL21 containing the λDE3 lysogen, which harbors…
BL21(DE3)pLysS vs. BL21(DE3) CodonPlus
Criteria BL21(DE3)pLysS BL21(DE3) CodonPlus Remarks Genetic Background Derivative of E. coli BL21(DE3) containing the pLysS plasmid Derivative of BL21(DE3) engineered…