Category: Lab Notes

Solute and solvent are the two components of the solution. The dissolved component is called solute whereas the component in…

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition sites Recognize hexameric palindromic sequence Blunt-end cutter (cut both…

Buffer Name pH range pKa at 25°C ACES 6.1 – 7.5 6.78  ADA 6.7 – 7.2  6.59  AMP 9.0 –…

Template independent DNA polymerase Also known as terminal transferase A member of the X family of DNA polymerase enzymes Add…

Formula: 1 molar (M) = 1000 millimolar (mM) More0.001 molar (M) = 1 millimolar (mM)0.01 molar (M) = 10 millimolar…

Formula: 1 milliliter (mL) = 1000 microliter (µL) More0.001 milliliter (mL) = 1 microliter (µL)0.01 milliliter (mL) = 10 microliter…

A 0.5M stock solution of Rubidium chloride (RbCl, Molecular weight = 120.92) can be prepared by dissolving 6.046 g RbCl in deionized water to a final volume of 100 ml.

TA cloning involves base pairing of adenine (A) and thymine (T)  (complementary base pairs) that result in the joining of…

T-vectors are suitable for cloning of DNA fragments that have been amplified by non-proofreading DNA polymerases. These vectors are linear…

IUPAC name 2-[(2-amino-2-oxoethyl)-(carboxymethyl)amino]acetic acid Synonym N-(2-Acetamido)iminodiacetic acid, N-(Carbamoylmethyl)iminodiacetic acid Molecular formula C6H10N2O5 Linear formula H2NCOCH2N(CH2CO2H)2 Molar mass 190.15 g/mol Appearance…

IUPAC name 2-(carbamoylmethylamino)ethanesulfonic acid Synonym N-(2-Acetamido)-2-aminoethanesulfonic acid Molecular formula C4H10N2O4S Molar mass 182.199 g/mol CAS number 7365-82-4 PubChem 81832 ChemSpider…