Category: Lab Notes

Deoxyribose

Molecular formula C5H10O4 Molecular weight 134.131 Molar mass  134.131g/mol IUPAC name  2-deoxy-d-ribose CAS Number  533-67-5 Chemically, Deoxyribose (or 2-deoxyribose) is…

Nitrogenous Bases

Nitrogenous bases (also known as Nucleobases or bases) are nitrogen-containing compounds. They are an important component of nucleic acids. These…

EcoRI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific cleavage sites Recognize hexameric palindromic sequence Sticky end cutter (cuts…

PmeI Restriction Enzyme

Synonyms: MssI Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize octameric palindromic sequence Blunt-end cutter (cut…

NheI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…

Ribose

Chemically, Ribose is a single-ring 5-carbon (pentose) sugar. Ribose is an essential component of ribonucleic acid (RNA). It can exist…

SmaI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Blunt-end cutter (cut both…

NotI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize octameric palindromic sequence Sticky end cutter (cut…

Protocol: Preparing T-vector using Terminal Transferase and ddTTP

T-vectors are linearized vectors that contain a single T-overhang at the 3’-end of both DNA strands. T-vectors are useful for…

XbaI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific sites Recognize hexameric palindromic sequence Sticky end cutter (cut both…

BamHI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…

KpnI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…

XhoI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…

Suppliers: 45% (w/w) Potassium Hydroxide Solution in Water

Note: A 45% (w/w) Potassium hydroxide solution can be purchased from the following suppliers. You must visit the supplier’s product…