Category: Lab Notes

Mycoplasma can affect cell culture in a variety of ways. These are: Morphological changes Slow proliferation rate Reduced saturation density…

Detection of mycoplasma is not as easy as detection of bacterial and yeast contamination. Mycoplasma contaminated cultures do not show…

Mycoplasma contamination is still a challenging problem in most cell culture laboratories. Due to their small size, they escape microscopic…

HCC827 – A Human epithelial-like lung adenocarcinoma (NSCLC) cell line, established from the lung of a white, 39-year-old female patient with adenocarcinoma.

To cryopreserve, cells from the suspension culture are harvested by centrifugation and resuspended at the correct cell density in an appropriate freezing medium. The resuspension is aliquoted into cryovials and subjected to slow freezing overnight followed by transfer to either the vapor phase of liquid nitrogen or a -150°C freezer. 

Cryopreservation enables long‑term storage of living cells and tissues at ultra‑low temperatures by halting biological time. This article explains freezing damage, cryoprotective agents, slow freezing, vitrification, and natural freeze tolerance.

Cell proliferation refers to a process of cell growth and cell division into two daughter cells. Cell proliferation leads to an exponential increase in cell number and tissue size.