NZ Amine A

NZ Amine is nothing but the end product obtained from enzymatic digestion of bovine milk protein casein. It is enriched…

Preparation of M9 Minimal Medium

OVERVIEW M9 minimal medium is a chemically-defined minimal growth medium, used to cultivate Escherichia coli. It is prepared by supplementing…

Elbing and Brent, 2019. Recipes and Tools for Culture of Escherichia coli. PMID-30412361

Elbing & Brent, 2019. Recipes and Tools for Culture of Escherichia coli. Curr Protoc Mol Biol. 125(1), e83. PMID-30412361; Full-Text…

Preparation of M63 Minimal Medium

OVERVIEW M63 Minimal Medium is a chemically-defined growth medium. It  can be used to culture Escherichia coli. It is prepared…

Preparation of 5x A Minimal Medium Without Supplements

OVERVIEW A Minimal Medium is a chemically-defined growth medium. It  can be used to culture Escherichia coli. It is prepared…

Preparation of 1 mM Biotin Stock Solution in PBS

OVERVIEW Biotin (also known as vitamin B7) is slightly soluble in water but soluble in hot and alkaline water. A…

Preparation of A Minimal Medium

OVERVIEW A Minimal Medium is a chemically-defined growth medium. It  can be used to culture Escherichia coli. It is prepared…

Preparation of 5x M63 Minimal Medium without supplements

OVERVIEW M63 Minimal Medium is a chemically-defined growth medium. It  can be used to culture Escherichia coli. It is prepared…

Preparation of 1 mg/ml Thiamine Stock Solution

OVERVIEW Thiamine, also known as vitamin B1, is soluble in water (50 mg/mL) and forms a clear colourless solution.  A…

Preparation of 1 mg/ml Biotin Stock Solution in Water

OVERVIEW Biotin (also known as vitamin B7) is slightly soluble in water but soluble in hot and alkaline water. A…

Growth of Escherichia coli in Liquid Medium

To grow Escherichia coli in liquid medium, a small amount of a growth medium (5ml or less) is inoculated with…

Escherichia coli serotype O157:H7

Overview: A member of Enterohaemorrhagic Escherichia coli gram negative, aerobic bacteria Major cause of food associated outbreaks Produces a potent…

Escherichia coli

Overview Rod-shaped, gram negative bacteria Extensively used in the research and biotech and pharmaceutical industry Most E. coli strains are…

Escherichia coli K-12 (MG1655) Complete Genome

Escherichia coli K-12 (MG1655) complete genome can be found in the link below or click here: https://www.ncbi.nlm.nih.gov/nuccore/U00096

Glioblastoma Stem Cells

It has been shown that Glioblastoma tumors have a subset of tumor cells that resembles closely to neural stem/progenitor cells….