Preparation of A Minimal Medium

OVERVIEW A Minimal Medium is a chemically-defined growth medium. It  can be used to culture Escherichia coli. It is prepared…

Preparation of 5x M63 Minimal Medium without supplements

OVERVIEW M63 Minimal Medium is a chemically-defined growth medium. It  can be used to culture Escherichia coli. It is prepared…

Preparation of 1 mg/ml Thiamine Stock Solution

OVERVIEW Thiamine, also known as vitamin B1, is soluble in water (50 mg/mL) and forms a clear colourless solution.  A…

Preparation of 1 mg/ml Biotin Stock Solution in Water

OVERVIEW Biotin (also known as vitamin B7) is slightly soluble in water but soluble in hot and alkaline water. A…

Growth of Escherichia coli in Liquid Medium

To grow Escherichia coli in liquid medium, a small amount of a growth medium (5ml or less) is inoculated with…

Escherichia coli serotype O157:H7

Overview: A member of Enterohaemorrhagic Escherichia coli gram negative, aerobic bacteria Major cause of food associated outbreaks Produces a potent…

Escherichia coli

Overview Rod-shaped, gram negative bacteria Extensively used in the research and biotech and pharmaceutical industry Most E. coli strains are…

Escherichia coli K-12 (MG1655) Complete Genome

Escherichia coli K-12 (MG1655) complete genome can be found in the link below or click here: https://www.ncbi.nlm.nih.gov/nuccore/U00096

Glioblastoma Stem Cells

It has been shown that Glioblastoma tumors have a subset of tumor cells that resembles closely to neural stem/progenitor cells….

Primary Brain Tumors

Primary brain tumors are tumors that arise within the brain or brain’s immediate surroundings. It can be any tissue located in the brain including central nervous system tissues (gliomas), pituitary gland (pituitary adenomas), pineal gland (pineocytomas), brain blood vessels (hemangioblastomas), etc.

Glioblastoma

Glioblastomas are highly malignant, Grade IV gliomas. They arise in the brain. Depending on their clinical history, they are of…

Anatomic Locations of Gliomas in Brain

The following article describes the anatomical locations of gliomas in the Finnish population. This study included 331 patients.  Larjavaara et…

Preparation of 10X TB salts

Overview: TB salt solution is nothing but a potassium phosphate buffer. A 10x TB salt solution contains 170 mM Potassium…

Preparation of SOC (Super Optimal broth with Catabolite repression) Medium

SOC (Super Optimal broth with Catabolite repression) medium is a nutrient-rich microbial growth medium, formulated by Douglas Hanahan in 1983 (Hanahan, 1983). SOC medium is prepared without Mg++ and Glucose by dissolving 20 g bacto-tryptone, 5 g bacto-yeast extract, 2 ml of 5M sodium chloride, and 2.5 ml of 1M KCl in water to a final volume of ≈ 1000 ml (960 ml). Magnesium salts (i.e., MgSO4 and MgCl2, 10 ml each of 1M MgSO4 and 1M MgCl2) and Glucose (10 ml of 1 M Glucose) are added to the medium after autoclaving to prevent any chemical reactions and precipitation of the medium components, and degradation of Glucose.

Syringe Filters

Syringe filters are disposable devices that are equipped with filter paper. These filter papers are available in different pore sizes,…