Tag: gene

trp Promoter

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The trp promoter is a naturally occurring promoter derived from the tryptophan (trp) operon of Escherichia coli, which controls genes…

T7 RNA Polymerase

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T7 RNA polymerase is a highly specific and efficient RNA polymerase derived from bacteriophage T7, widely used in molecular biology…

In Vitro T7 Transcription System

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The in vitro T7 transcription system is a widely used tool in molecular biology that harnesses the powerful and specific…

pLysS System Vs pLysE System

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Criteria pLysS System pLysE System Remarks Encoded Protein T7 Lysozyme (low expression level) T7 Lysozyme (high expression level) Both express…

SP6 RNA Polymerase

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SP6 RNA polymerase is a DNA-dependent RNA polymerase derived from Salmonella bacteriophage SP6, commonly used for in vitro transcription of…

T3 RNA Polymerase

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T3 RNA polymerase is a DNA-dependent RNA polymerase derived from bacteriophage T3, closely related to T7 RNA polymerase, but with…

T3 Promoter

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The T3 promoter is a highly specific DNA sequence recognized exclusively by T3 RNA polymerase, originating from bacteriophage T3. It…

SP6 Promoter

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The SP6 promoter is a short, well-characterized DNA sequence recognized specifically by SP6 RNA polymerase, derived from Salmonella bacteriophage SP6. …

Ptac Promoter

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The Ptac promoter is a synthetic hybrid promoter created by combining elements of the trp and lac promoters, designed to…

araBAD Promoter

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The araBAD promoter is a tightly regulated and inducible promoter derived from the arabinose operon of Escherichia coli, which controls…

T7 Promoter

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The T7 promoter is a bacteriophage-derived promoter that is widely used for high-level expression of recombinant proteins in Escherichia coli…

LacUV5 Promoter

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The lacUV5 promoter is a mutated version of the native E. coli lac promoter, engineered for stronger and more consistent…

Escherichia coli BL21(DE3)pLysS

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Escherichia coli (E. coli) BL21(DE3)pLysS is a widely used strain for controlled recombinant protein expression, particularly when working with proteins…

Escherichia coli BL21

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Escherichia coli (E. coli) BL21 is one of the most commonly used bacterial strains for recombinant protein expression.  Derived from…

Escherichia coli Expression System

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The Escherichia coli (E. coli) expression system is one of the most widely used platforms for recombinant protein production in…