Tag: plasmid isolation

Solution I for Plasmid Isolation by Alkaline Lysis Method

Preparation of Resuspension Buffer Containing Tris and EDTA for Isolation of Plasmid by Alkaline Lysis Method

 admin  September 5, 2020  2 Comments on Preparation of Resuspension Buffer Containing Tris and EDTA for Isolation of Plasmid by Alkaline Lysis Method

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Overview: The resuspension buffer is used to resuspend bacterial cells during plasmid isolation by the alkaline lysis method. It provides…

Basic Steps in Plasmid Isolation

 admin  September 3, 2020  1 Comment on Basic Steps in Plasmid Isolation

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Plasmid isolation is a common task in molecular biology laboratories. Tasks as simple as amplifying a plasmid vector to complex…

Protocol – Plasmid Isolation by Alkaline Lysis Method (Miniprep)

 admin  August 11, 2020  1 Comment on Protocol – Plasmid Isolation by Alkaline Lysis Method (Miniprep)

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OVERVIEW The alkaline lysis method of plasmid isolation was originally developed by Brinboim and Doly (1979). In this procedure, bacteria…