Topic: Cell Culture

• Cell Culture  
• Animal Cell Culture
• Cell Culture: Timeline of Key Events  
  • The Origin of Cell Culture: Ross Harrison’s Pioneering 1907 Experiment
• Dynamic Nature of Cells in Culture
• Passage Number  
• Primary Culture
  • Challenges in Primary Tissue Processing for Cell Isolation  
  • Immortalized Cell Lines as Alternatives to Primary Cultures
• Cell Immortalization  
  • Cell Immortalization – Timeline of Key Events
  • Immortalized Cell Line  
  • Advantages and Limitations of Immortalized Cell Lines  
  • Applications of Immortalized Cell Lines
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Cell culture handling and maintenance

• Preparing Laminar Flow Hood for Cell Culture Work
• Feeding Cells   
  • Protocol – Feeding Adherent Culture   
  • Protocol – Feeding Suspension Culture (Partial Replacement of Medium)
  • Protocol – Feeding Suspension Culture (Complete Replacement of Medium)
• Passaging/subculturing cells   
• Passaging/Subculturing Methods for Cells   
• Protocol – Passaging/Subculturing Suspension Culture   
• Protocol – Subculturing Adherent Cells Growing in Serum-containing Growth Medium using Trypsin-EDTA   
• Aspirating Medium from Adherent Culture   

Cell Culture Contamination

• Cell Culture Contamination  
• Bacterial Contamination in Cell Culture
• Mycoplasma Contamination in Cell Culture   
  • Contaminating Mycoplasma species in Cell Culture  
  • Common Mycoplasma Species Detected in Cell Culture
  • Sources of Mycoplasma Contamination  
  • Detection of Mycoplasma Contamination
  • Preventing Mycoplasma Contamination in Cell Culture  
  • Curing Mycoplasma Infected Culture   

Reagents and solutions

• Protocol – Heat Inactivation of Serum   
• Preparation of Stock Solution of L-Glutamine (200 mM)   
• Handling and Aliquoting Frozen Stock of Serum
• Cell Culture Antibiotics  
  • List of Commonly Used Antibiotics in Cell Culture   
  • Penicillin in Cell Culture   
  • Impact of Penicillin on Mitochondria in Mammalian Cell Cultures   
  • Gentamicin in Cell Culture    
  • Impact of Gentamicin on Mitochondria in Mammalian Cell Cultures    
  • Streptomycin in Cell Culture    
  • Impact of Streptomycin on Mitochondria in Mammalian Cell Cultures

Instrumentation and disposables

• Commonly Used Cell Culture Vessels
• T-225 Flasks for Cell Culture
• T-175 flasks for cell culture
• T-75 Flasks for Cell Culture
• T-25 Flasks for Cell Culture

Related topics

• Protocol – RNA Isolation from Tissue Using Trizol
• Protocol – RNA Isolation from Suspension Cell Culture Using Trizol
• Preparation of Metaphase Chromosome Spreads from Adherent Cell Line 
• How To Choose Appropriate Cell Line For Research?
• Topic: Cryopreservation