Category: Lab Notes: Microscopy
CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis)
CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) is a comprehensive tissue clearing method that uses simple, hydrophilic chemicals…
DISCO (Tissue Clearing Method)
DISCO (3DISCO, iDISCO, uDISCO) represents a family of organic solvent-based tissue clearing methods that have revolutionized the way researchers visualize…
SCALE (Sorbitol/Calf serum/Alpha-lipoic acid/Ellipsenone)
SCALE (Sorbitol/Calf serum/Alpha-lipoic acid/Ellipsenone) is a hydrophilic tissue clearing method that achieves transparency through hyperhydration while preserving fluorescent proteins and…
SeeDB (See Deep Brain)
SeeDB (See Deep Brain) is an optical clearing method that utilizes simple sugar solutions to render biological tissues transparent while…
Serial Two-Photon Tomography
Serial Two-Photon Tomography (STPT) is an advanced imaging technique that combines the high-resolution, depthpenetra-ting capabilities of two-photon microscopy with automated…
Optical Projection Tomography
Optical Projection Tomography (OPT) is a powerful imaging technique used to acquire detailed three-dimensional (3D) representations of biological samples, particularly…
Swept Confocally-Aligned Planar Excitation (SCAPE) Microscopy
Swept Confocally-Aligned Planar Excitation (SCAPE) microscopy is an advanced light-sheet imaging technique designed to rapidly capture high-resolution, volumetric data from…
Volume Imaging Electron Microscopy
Volume imaging electron microscopy refers to a collection of advanced electron microscopy (EM) techniques that enable the reconstruction of three-dimensional…
Oblique Plane Microscopy
Oblique Plane Microscopy (OPM) is an innovative fluorescence imaging technique that enables rapid, three-dimensional imaging of living specimens by illuminating…
Two-Photon Microscopy
Two-photon microscopy is an advanced fluorescence imaging technique that enables deep-tissue visualization with high spatial resolution and minimal photodamage. It…
Super-Resolution Microscopy
Super-resolution microscopy encompasses a group of advanced optical imaging techniques that surpass the resolution limits of conventional light microscopy, enabling…
Widefield Microscopy
Widefield microscopy is one of the most widely used and foundational techniques in optical microscopy. It involves illuminating the entire…
Laser Scanning Confocal Microscopy
Laser scanning confocal microscopy (LSCM) is a powerful fluorescence imaging technique that enables the acquisition of high-resolution, optically sectioned images…
Laser Scanning Vs Spinning Disk Confocal Microscopy
Criteria Laser Scanning Confocal Microscopy (LSCM) Spinning Disk Confocal Microscopy Remarks Principle Uses a single point laser beam scanned across…
Spinning Disk Confocal Microscopy
Spinning disk confocal microscopy is an advanced imaging technique that enables rapid and high-resolution three-dimensional imaging of fluorescently labeled specimens,…