- Cell Line: HCC4006
- Synonyms:
- HCC-4006
- Hamon Cancer Center 4006
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cancer Cell Line
- Description: Established from pleural effusion of the lung of a white, adult male patient with adenocarcinoma.
- Origin:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- ≈ 41 hrs (ATCC)
- 46.8 hours (cellosaurus)
- Gene expression:
- Sequence variations (gene mutations/deletions/fusions/translocations):
- EGFR (L747 – E749 deletion, A750P mutation in tyrosine kinase domain)
- TP53 (Y205H mutation, Likely Loss-of-function) (depmap)
- For more information, please visit
- https://depmap.org/portal/cell_line/ACH-000066?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000066?tab=fusions
- https://depmap.org/portal/cell_line/ACH-000066?tab=translocations
- EGFR (L747 – E749 deletion, A750P mutation in tyrosine kinase domain)
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: sub-confluent culture
- Split ratio: 1:4 to 1:6
- Medium Renewal: Every 2 to 3 days (depending on cell density)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 – 5 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2871
- https://www.cellosaurus.org/CVCL_1269
- https://depmap.org/portal/cell_line/ACH-000066?tab=overview
- Shigematsu et al. 2005. Somatic mutations of the HER2 kinase domain in lung adenocarcinomas. Cancer Res. 65, 1642-1646. PMID-15753357; Full Text: aacrjournals
- Images
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- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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