- Cell Line: HCC70
- Synonyms:
- HCC-70
- HCC 70
- HCC0070
- Hamon Cancer Center 70
- Culture Type: Cell line
- Keywords: Human mammary gland epithelial-like, PR negative, HER2 negative ductal carcinoma cell line
- Description: Established from the primary tumor cells of a 49-year-old black woman with breast carcinoma (ductal type; TNM stage IIIA, grade 3)
- Origin:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Polyploid
- Doubling time:-
- Clinically relevant genes:
- her2/neu negative (oncogene)
- p53 positive, overexpressed (oncogene)
- Estrogen receptor (ER) negative (ATCC)
- Progesterone receptor (PR) negative
- Epithelial cell-specific marker gene expression
- Epithelial glycoprotein 2 (EGP2) positive
- Cytokeratin 19 positive
- Epithelial glycoprotein 2 (EGP2) positive
- Sequence variations (gene mutations/deletions/fusions/translocations):
- TP53 (R248Q mutation, Likely Loss-of-function) (depmap)
- For more information, please visit
- TP53 (R248Q mutation, Likely Loss-of-function) (depmap)
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time: sub-confluent culture
- Split ratio: 1:4 to 1:6
- Medium Renewal: Every 2 to 3 days (depending on cell density)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density): 2 – 5 x 106 cells/ml
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- BCRJ – Banco de Células do Rio de Janeiro (Cell Bank of Rio de Janeiro): Product link
- References
- https://www.atcc.org/products/crl-2315
- https://www.cellosaurus.org/CVCL_1270
- https://depmap.org/portal/cell_line/ACH-000668?tab=overview
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Images
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- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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