- Cell Line: NCI-H1299
- Synonyms:
- NCI-H1299
- NCIH1299
- H1299
- H-1299
- Culture Type: Cell line
- Keywords: Human Large Cell Lung Carcinoma Epithelial Cell Line
- Description: Established from the Lymph node metastatic tumor of a 43-year-old, Caucasian male patient with Large Cell Lung Carcinoma.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 43 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lymph node (metastatic site)
- Disease:
- Large Cell Lung Carcinoma
- Primary/Metastasis: Metastatic
- Large Cell Lung Carcinoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:
- 22.5 hours (McMillan et al., 2018). (Cellosaurus)
- 30 hours (Cowley et al., 2014) (Cellosaurus)
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:- Yes, cells produce tumors in athymic nude mice
- Gene expression:
- Positive for keratin
- Positive for vimentin
- Negative for neurofilament triplet protein
- Negative for p53 protein
- Sequence variations:
- Homozygous partial deletion of the p53 protein
- NRAS (Q61K mutation, Oncogenic, Gain-of-function, Heterozygous)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Immuno-oncology research
- Suitable transfection host
- Availability
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- BCRJ (Banco de Células do Rio de Janeiro): Product link
- References
- Images:
- NCI-H1299 cell image link (Source: ATCC)
- NCI-H1299 cell image link (Source: DepMap)
- NCI-H1299 cell image link (Source Cytion)
- NCI-H1299 cell image link (Source Cytion)
- NCI-H1299 cell image link (Source Cytion)
- NCI-H1299 cell image link (Source Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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