- Cell Line: NCI-H1437
- Synonyms:
- NCI-H1437
- NCIH1437
- H1437
- H-1437
- Culture Type: Cell line
- Keywords: Human non-small cell lung cancer cell line
- Description: Established from pleural effusion metastasis of lung from a 60-year-old, White male with stage 1 non-small cell lung cancer
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 60 years
- Ethnicity: White
- Gender: Male
- Smoking habit:-
- Tissue (origin): Lung
- Disease: Non-small cell lung cancer, Stage 1 (Lung Adenocarcinoma)
- Primary/Metastasis: Metastatic (Collection Site: pleural effusion)
- Year of origin: 1986
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time: Approximately 44 hrs - Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (R267P mutation, Likely Oncogenic, Likely Gain-of-function, Homozygous)
- MAP2K1 (Q56P mutation, Oncogenic, Gain-of-function)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000589?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000589?tab=fusion
- https://depmap.org/portal/cell_line/ACH-000589?tab=translocations
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Semi confluent culture (~ 80%)
- Seeding density:-
- Recommended split ratio: 1:2 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- Cytion: Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- Article Authenticity Index *
- Please let us know if you find any mistakes. Your comments are welcome in the comment box.
