- Cell Line: NCI-H1781
- Synonyms:
- NCI-H1781
- NCIH1781
- H1781
- H-1781
- Culture Type: Cell line
- Keywords: Human Lung Bronchoalveolar Carcinoma Cell Line
- Description: Established from the lungs of a 66-year-old, Caucasian female with bronchoalveolar carcinoma, stage 3B
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 66 years
- Ethnicity: Caucasian
- Gender: Female
- Smoking habit: Smoker
- Tissue (origin): Lung
- Disease: Adenocarcinoma, Bronchoalveolar carcinoma, Stage 3B
- Primary/Metastasis: Metastatic
- Collection site: Pleural effusion
- Year of origin: 1987
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:
- Doubling time:-
- Microsatellite instability: Instable
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- TP53 (V157F mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000379?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000379?tab=fusions
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% fetal bovine serum
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.03 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2 to 1:4
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% (v/v) DMSO
- Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- Cancer research
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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