- Cell Line: NCI-H209
- Synonyms:
- NCI-H209
- NCIH209
- H209
- H-209
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell Line
- Description: Established from the bone marrow of a 55-year-old caucasian male with small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 55 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Bone marrow
- Disease:
- Small cell lung carcinoma
- Primary/Metastasis: Metastatic (Bone marrow)
- Year of origin: 1979
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Rounded, large aggregates
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Hyperdiploid, Modal chromosome number – 49
- Doubling time:
- 50-70 hours
- 50-70 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity: Tumorigenic in nude mice
- Gene expression:
- Cells are reported to express neuroendocrine biochemical markers such as
- neuron-specific enolase
- brain creatine kinase
- L-DOPA decarboxylase
- bombesin
- Positive for vimentin
- Negative for neurofilament
- Negative for L-dopa decarboxylase
- Cells are reported to express neuroendocrine biochemical markers such as
- Sequence variations:
- RB1 (C706F mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- RB1 (C706F mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Harvesting time:-
- Split ratio: 1:2 to 1:3
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Applications:
- Cancer research
- 3D cell culture
- Immunology
- Availability:
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- AcceGen: Cat.No ABC-TC603S
- References
- Images:
- NCI-H209 cell image link (Source: DSMZ)
- NCI-H209 cell image link (Source: DSMZ)
- NCI-H209 cell image link (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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