- Cell Line: NCI-H2171
- Synonyms:
- NCI-H2171
- NCIH2171
- H2171
- H-2171
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from the pleural effusion metastasis of a 50-year-old white male patient with small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 50 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion
- Disease: Small Cell Lung Cancer, Stage E
- Primary/Metastasis: Metastatic (Pleural effusion)
- Year of origin: 1989
- Organism:
- Cell line features:
- Cell Type: Lymphoblast (ATCC)
- Morphology: Floating clusters
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Human hypodiploid karyotype with 8% polyploidy
Doubling time: ~35-60 hours (DSMZ) - Microsatellite instability: Instable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- Cell Type: Lymphoblast (ATCC)
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- RPMI 1640 + 20% heat-inactivated FBS (DSMZ)
- DMEM:F12 Medium (ATCC, Catalog No. 30-2006) + Supplements (ATCC)
- Supplements
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc)
- Supplements
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Harvesting time: 2 – 3.0 x 106 cells/ml
- Seeding density: 1.0 x 106 cells/ml
- Split ratio: 1:2 to 1:3
- Medium Renewal: Every 2 to 3 days
- Subculturing method: Dilution
- Cryopreservation:
- Applications:
- Cancer research
- 3D cell culture
- Immunology
- Availability:
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures GmbH: Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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