- Cell Line: NCI-H2171
- Synonyms:
- NCI-H2227
- NCIH2227
- H2227
- H-2227
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from the lungs of a 54-year-old, white male patient with stage E small cell lung carcinoma.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 54 years
- Ethnicity: White
- Gender: Male
- Tissue (origin): Lung
- Disease: Small Cell Lung Cancer, Stage E
- Primary/Metastasis: Primary
- Year of origin: 1989
- Organism:
- Cell line features:
- Cell Type:-
- Morphology:-
- Growth mode: Mixed: adherent and suspension
- Life span: Infinite (continuous culture)
- Karyotype:-
Doubling time:- - Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:-
- Sequence variations:
- PDGFRA (P577T mutation, Likely Oncogenic, Likely Gain-of-function)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- DMEM:F12 Medium (ATCC, Catalog No. 30-2006) + Supplements
- Supplements
- 0.005 mg/ml Insulin
- 0.01 mg/ml Transferrin
- 30nM Sodium selenite (final conc.)
- 10 nM Hydrocortisone (final conc.)
- 10 nM beta-estradiol (final conc.)
- extra 2mM L-glutamine (for final conc. of 4.5 mM)
- 5% fetal bovine serum (final conc)
- Supplements
- DMEM:F12 Medium (ATCC, Catalog No. 30-2006) + Supplements
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution (suspension cells) + mechanical means (scrapping of adherent cells)
- Harvesting time:-
- Seeding density:-
- Split ratio:-
- Medium Renewal: Two to three times weekly
- Subculturing method: Dilution (suspension cells) + mechanical means (scrapping of adherent cells)
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% FBS + 5% DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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