- Cell Line: NCI-H23
- Synonyms:
- NCI-H23
- NCIH23
- H23
- H-23
- Culture Type: Cell line
- Keywords: Human Non-small Cell Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the primary lung tumor of a 51-year-old, African American male patient with Non-small cell lung adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 51 years
- Ethnicity: African American
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Lung (tumor tissue)
- Disease:
- Non-small cell lung adenocarcinoma
- Primary/Metastasis: Primary
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:
- ≈ 38 hours
- ≈ 38 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:
- Positive for keratin (5, 8, 18)
- Positive for vimentin
- Negative for neurofilament
- Negative for L-dopa decarboxylase
- Positive for keratin (5, 8, 18)
- Sequence variations:
- KRAS (G12C mutation, Oncogenic, Gain-of-function, Heterozygous)
- TP53 (M246I mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.03 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:6
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- High-throughput screening
- Toxicology
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H23 cell image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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