- Cell Line: NCI-H295R
- Synonyms:
- NCI-H295R
- NCIH295R
- H295R
- H-295R
- Culture Type: Cell line
- Keywords: Human Adrenocortical Carcinoma Cell Line
- Description:
- Parent cell line: NCI-H295
- The parent cell line was adapted to a culture medium that decreased the population doubling time from 5 days to 2 days.
- While NCI-H295 grows in suspension, the NCI-H295R grows as an adherent monolayer.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 48 years
- Ethnicity: Black
- Gender: Female
- Tissue (origin): Adrenal gland, Cortex
- Disease:
- Adrenal cortex carcinoma
- Primary/Metastasis: Primary
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: 25 hours
- Microsatellite instability:-
- Special features:-
- Product: Adrenal androgens
- Tumorigenicity:-
- Gene expression:
- Positive for aldosterone
- Positive for cortisol
- Positive for C19 steroids
- Sequence variations:-
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium + 0.00625 mg/ml insulin + 0.00625 mg/ml transferrin + 6.25 ng/ml selenium + 1.25 mg/ml bovine serum albumin + 0.00535 mg/ml linoleic acid + 2.5% Nu-Serum I (final concentration). (ATCC)
- Note: The additives (insulin, transferrin, selenium, BSA and linoleic acid) are available in the form of ITS+ Premix from Corning (Catalog No. 354352). Nu-Serum is also available from Corning (Catalog No. 355100) (ATCC)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:4
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freezing medium: Complete growth medium + additional 7.5% Nu-Serum + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- NCI-H295R cell image link (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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