- Cell Line: NCI-H441
- Synonyms:
- NCIH441
- NCI-H441-4
- H441
- H-441
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the pericardial fluid of an adult White male patient with papillary adenocarcinoma of the lung
- Origin:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Hyperdiploid human cell line, modal number = 52; range = 44 to 59
- Doubling time: ≈ 58 h (in standard culture medium)
- Microsatellite instability: Stable (cellosaurus)
- Special features:-
- Product:-
- Tumorigenicity:-
- Gene expression:
- Positive for mRNA and protein of the major surfactant apoprotein (SP-A)
- Positive for mRNA and protein of the major surfactant apoprotein (SP-A)
- Sequence variations:
- KRAS (G12V mutation, Oncogenic, Gain-of-function)
- TP53 (R158L mutation, Likely Oncogenic, Likely Loss-of-function)
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- Cell Type: Epithelial cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:8
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- High-throughput screening
- Toxicology
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- ATCC
- Cellosaurus
- Depmap
- Bepler et al., 1989. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50. PMID-2536917
- Images:-
- NCI-H441 cell image (Source: ATCC)
- NCI-H441 cell image (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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