- Cell Line: NCI-H929
- Synonyms:
- NCIH929
- NCI H929
- H929
- H-929
- Culture Type: Cell line
- Keywords: Human Plasma Cell Myeloma cell line
- Description: Established from the pleural effusion of a 62-year-old white woman with plasmacytoma myeloma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 62 years
- Ethnicity: White
- Gender: Female
- Tissue (origin): Bone marrow
- Tissue (isolation): pleural effusion
- Disease:
- Plasma Cell Myeloma
- Molecular subtype: Plasmacytoma
- Primary/Metastasis: Metastatic (depmap)
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: B lymphocyte
- Morphology: Round to oval cells
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Near tetraploid
- Doubling time: ≈70 h
- Product: Produces IgA kappa
- Gene expression:
- Positive for PCA-1
- Positive for CD38 (10)
- Positive for transferrin receptor
- Negative for markers of early B cell development
- Negative for Epstein-Barr virus nuclear antigen (EBNA)
- Express c-myc RNA
- Sequence variations:
- NRAS (G13D mutation, Oncogenic Gain-of-function)
- Rearrangement of the c-myc proto-oncogene
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- Cell Type: B lymphocyte
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 0.05 mM 2-mercaptoethanol + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Maintain cultures at cell concentrations between 5 x 105 and 1 x 106 viable cells/mL
- Split ratio:-
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium: Complete growth medium + 20% FBS + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):- 5 x 106 cells
- Volume per vial: 1 mL
- Applications:
- Cancer research
- Immunology research
- Cancer research
- Availability
- American Type Culture Collection (ATCC): Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures: Product link
- ITI BioChem: Product link
- References
- Images:-
- NCI-H929 cell image 1 (Source: DSMZ)
- NCI-H929 cell image 2 (Source: DSMZ)
- NCI-H929 cell image 3 (Source: DSMZ)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
- PCA-1 (plasma cell antigen 1)
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