- Cell Line: NCI-N87
- Synonyms:
- NCIN87
- NCI-N87
- N87
- N-87
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Epithelial Cell Line
- Description: Established from the metastatic tumor (liver metastasis) of a caucasian, male patient with Gastric tubular adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity:-
- Gender: Male
- Tissue (origin): Stomach
- Tissue (isolation): Liver (metastatic site)
- Disease:
- Stomach Adenocarcinoma
- Molecular subtype: Tubular
- Primary/Metastasis: Metastasis
- Stomach Adenocarcinoma
- Year of origin: 1976
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Karyotype: Near diploid
- Doubling time: 47 hours
- Microsatellite instability: Stable
- Special features:-
- Product:-
- Tumorigenicity: Cells form tumors in athymic nude mice
- Gene expression:-
- positive for myc and erb-B2
- Express Acetylcholine and muscarinic
- Sequence variations:
- TP53 (R248Q mutation, Likely Oncogenic, Likely Loss-of-function)
- For information, please visit
- Cell Type:-
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:3 to 1:4
- Medium Renewal: Two to three times weekly
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- Cyton: Product number: Product link
- References
- Images:-
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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