- Cell Line: Panc 10.05
- Synonyms:
- PANC1005
- PANC 1005
- Panc1005
- Panc-10.05
- Panc10.05
- PANC-10-05
- Culture Type: Cell line
- Keywords: Human pancreatic adenocarcinoma epithelial cell line
- Description: Established from the primary tumor taken from a White adult male with pancreatic adenocarcinoma.
- Origin:
- Cell line features:
- Cell Type: Epithelial cell
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ≈19.2 hrs
- Product:
- Tumorigenicity: Yes, forms tumors in nude or SCID mice
- Gene expression:
- Positive for K-ras (Oncogene)
- Positive for MHC class I (Antigen)
- Negative for MHC class II (Antigen)
- Positive for cytokeratin 7
- Positive for cytokeratin 18
- Positive for K-ras (Oncogene)
- Sequence variations:
- KRAS (G12D mutation, Oncogenic Gain-of-function)
- TP53 (I255N mutation, Likely oncogenic Likely Loss-of-function)
- For more information, please visit
- Cell Type: Epithelial cell
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10 Units/ml human recombinant insulin + 15% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:-
- Split ratio: 1:2 to 1:4
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Applications:
- Cancer research
- 3D cell culture
- Cancer research
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- ATCC
- Cellosaurus
- Depmap
- Jaffee et al., 1998. Development and characterization of a cytokine-secreting pancreatic adenocarcinoma vaccine from primary tumors for use in clinical trials. Cancer J. Sci. Am. 4: 194-203. PMID-9612602
- Images:-
- Panc 10.05 cell image (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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