- Cell Line: SW-1116
- Synonyms:
- SW-1116
- SW 1116
- SW1116
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell Line
- Description: Established from a 73-year-old Caucasian male patient with Colorectal adenocarcinoma
- Origin:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: hypotriploid
- Doubling time: ~ 68.70 hours (cellosaurus)
- Microsatellite instability: Stable
- Special features: positive for keratin by immunoperoxidase staining
- Product: Carcinoembryonic antigen (CEA)
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression:-
- Sequence variations:
- KRAS (G12A mutation, Oncogenic, Gain-of-function, Heterozygous)
- TP53 (A159 mutation, Likely Oncogenic, Likely Loss-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000489?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000489?tab=fusions
- KRAS (G12A mutation, Oncogenic, Gain-of-function, Heterozygous)
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium:
- Leibovitz’s L-15 Medium + 10% FBS
- Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:3 to 1:6
- Medium Renewal: 1 to 2 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO
- Applications:
- 3D cell culture
- Cancer research
- High-throughput screening
- Toxicology
- Cell Line Availability: The cell line can be obtained from the following sources.
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- Cytion: Product link
- Sigma Aldrich: Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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