- Cell Line: SW-620
- Synonyms:
- SW-620
- SW 620
- SW620
- Culture Type: Cell line
- Keywords: Human Colorectal Adenocarcinoma Cell Line
- Description: Established from a 51 years old Caucasian male patient with Dukes C colorectal adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 51 years
- Ethnicity: Caucasian
- Gender: Male
- Tissue: Large intestine, Colon
- Disease: Colorectal adenocarcinoma Dukes’ type C
- Primary/Metastasis: Metastatic
- Collection site: Lymph node
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like (small spherical and bipolar cells lacking microvilli)
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Modal number = 50, range = 45 to 53
- Doubling time: ~26 hours (Cellosaurus)
- Microsatellite instability: stable
- Special features:-
- Product: Carcinoembryonic antigen (CEA)
- Soft agar colony formation assay:-
- Tumorigenicity: tumorigenic in nude mice
- Gene expression: keratin positive by immunoperoxidase staining
- Sequence variations:
- TP53 (R273H mutation, Oncogenic, Loss-of-function, Heterozygous)
- KRAS (G12V mutation, Oncogenic, Gain-of-function, Homozygous)
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-000651?tab=mutations
- https://depmap.org/portal/cell_line/ACH-000651?tab=fusions
- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level:
- BSL 1 (ATCC)
- BSL 2 (Culturecollections)
- It may differ in your country, follow the guidelines of your institute/country
- Complete culture medium: Leibovitz’s L-15 Medium + 10% FBS
- Culture conditions:
- Temperature: 37°C incubator
- Atmosphere: 100% Air
- Temperature: 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin- 0.53 mM EDTA solution
- Harvesting time:-
- Seeding density:-
- Recommended split ratio: 1:2 to 1:10
- Medium Renewal: 2 to 3 times per week
- Subculturing method: Enzymatic dissociation
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium: Complete growth medium + 5% (v/v) DMSO (ATCC)
- Biosafety level:
- Applications:
- High-throughput screening
- 3D cell culture
- Toxicology
- transfection host
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- Cytion: Product link
- References
- Images:
- SW-620 cell line image link 1 (Source: DepMap)
- SW-620 cell line image link 2 (Source: Cytion)
- SW-620 cell line image link 3 (Source: Cytion)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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