KpnI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…

XhoI Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…

Suppliers: 45% (w/w) Potassium Hydroxide Solution in Water

Note: A 45% (w/w) Potassium hydroxide solution can be purchased from the following suppliers. You must visit the supplier’s product…

Suppliers: 95% (w/w) Sulfuric Acid Solution

Note: A 95% (w/w) Sulfuric acid  solution can be purchased from the following suppliers. You must visit the supplier’s product…

Solute

Solute and solvent are the two components of the solution. The dissolved component is called solute whereas the component in…

EcoRV Restriction Enzyme

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition sites Recognize hexameric palindromic sequence Blunt-end cutter (cut both…

Biological Buffers

Buffer Name pH range pKa at 25°C ACES 6.1 – 7.5 6.78  ADA 6.7 – 7.2  6.59  AMP 9.0 –…

Terminal Deoxynucleotidyl Transferase (TdT)

Template independent DNA polymerase Also known as terminal transferase A member of the X family of DNA polymerase enzymes Add…

Molar – Millimolar Conversion

Formula: 1 molar (M) = 1000 millimolar (mM) More0.001 molar (M) = 1 millimolar (mM)0.01 molar (M) = 10 millimolar…

Calculator: Milliliter – Microliter Conversion

Formula: 1 milliliter (mL) = 1000 microliter (µL) More0.001 milliliter (mL) = 1 microliter (µL)0.01 milliliter (mL) = 10 microliter…

How to Study Changes in Buffer pH During Agarose Gel Electrophoresis?

Changes in electrophoresis buffer pH is an important parameter to consider when optimizing already existing reagents or conditions or testing…