Laboratory Notes

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition site Recognize hexameric palindromic sequence Sticky end cutter (cut…

Note: A 45% (w/w) Potassium hydroxide solution can be purchased from the following suppliers. You must visit the supplier’s product…

Note: A 95% (w/w) Sulfuric acid  solution can be purchased from the following suppliers. You must visit the supplier’s product…

Solute and solvent are the two components of the solution. The dissolved component is called solute whereas the component in…

Endonuclease Type II Restriction enzyme Cleaves double-stranded DNA at specific recognition sites Recognize hexameric palindromic sequence Blunt-end cutter (cut both…

Buffer Name pH range pKa at 25°C ACES 6.1 – 7.5 6.78  ADA 6.7 – 7.2  6.59  AMP 9.0 –…

Template independent DNA polymerase Also known as terminal transferase A member of the X family of DNA polymerase enzymes Add…

Formula: 1 molar (M) = 1000 millimolar (mM) More0.001 molar (M) = 1 millimolar (mM)0.01 molar (M) = 10 millimolar…

Formula: 1 milliliter (mL) = 1000 microliter (µL) More0.001 milliliter (mL) = 1 microliter (µL)0.01 milliliter (mL) = 10 microliter…

Changes in electrophoresis buffer pH is an important parameter to consider when optimizing already existing reagents or conditions or testing…