- The pSilencer™ 2.1-U6 Hygro is a plasmid vector specifically designed for RNA interference (RNAi) applications in mammalian cells.
- It facilitates the expression of short hairpin RNA (shRNA) under the control of the U6 RNA polymerase III promoter, enabling efficient and stable gene silencing.
- It is widely used to generate stable knockdown cell lines.
- It is commercially available from Thermo Fisher Scientific (Ambion™).
- It includes a hygromycin resistance gene, which allows for the selection of stable transfectants in mammalian cells, ensuring the long-term maintenance of gene silencing effects.
- The vector carries an ampicillin resistance gene, which facilitates bacterial selection during plasmid propagation in E. coli.
- A multiple cloning site (MCS) is present in the vector, allowing for the easy insertion of target-specific shRNA sequences. For sequencing and verification of the cloned fragments, the T7 and 2.0rev primers are used.
- The ColE1 origin of replication (ColE1 ori) serves as the starting point for plasmid DNA replication in bacterial host cells, primarily in E. coli, ensuring high-copy plasmid amplification for further applications.
- This vector is optimized for both transient and stable RNAi experiments, offering high efficiency and reliable gene silencing.
- It is suitable for a wide range of applications, including gene function studies, therapeutic research, and the development of stable RNAi cell lines.
- Links:
Disclaimer: While we strive to continuously improve and update our content, we cannot guarantee its accuracy or completeness. Please note that the content is continuously evolving, and updates may not reflect the most current information. The information contained on this website is for general informational purposes only and should not be considered as professional advice. We disclaim any liability for any loss or damage resulting from the use of the information provided herein. Always consult qualified professionals for specific guidance and verify any critical information before relying on it. Read more
