Tag: escherichia coli
BL21 Vs BL21(DE3) Vs BL21(DE3)pLysS
Criteria BL21 BL21(DE3) BL21(DE3)pLysS Remarks Genetic Background Wild-type E. coli B strain derivative BL21 containing the λDE3 lysogen, which harbors…
BL21(DE3)pLysS vs. BL21(DE3) CodonPlus
Criteria BL21(DE3)pLysS BL21(DE3) CodonPlus Remarks Genetic Background Derivative of E. coli BL21(DE3) containing the pLysS plasmid Derivative of BL21(DE3) engineered…
Escherichia coli SHuffle
Escherichia coli SHuffle is a specially engineered strain developed to enhance the correct folding of disulfide bond-containing proteins in the…
Escherichia coli Rosetta
Escherichia coli Rosetta is a genetically engineered strain designed to enhance the expression of eukaryotic proteins in bacterial systems by…
Escherichia coli BL21(DE3)
Escherichia coli (E. coli) BL21(DE3) is one of the most widely used bacterial strains for recombinant protein expression due to…
Escherichia coli BL21(DE3) CodonPlus
Escherichia coli (E. coli) BL21(DE3) CodonPlus is a specialized derivative of the BL21(DE3) strain designed to improve the expression of…
Escherichia coli BL21(DE3)pLysS
Escherichia coli (E. coli) BL21(DE3)pLysS is a widely used strain for controlled recombinant protein expression, particularly when working with proteins…
pEC156
pEC156 is a naturally occurring plasmid found in Escherichia coli, classified as a small, high-copy-number, non-conjugative but mobilizable plasmid. With…
Escherichia coli Pathogenic Strains
Pathogenic strains of Escherichia coli represent distinct variants that have acquired specific virulence factors through horizontal gene transfer and evolutionary…
Escherichia coli in Research: Timeline of Discovery and Innovation
1885: Theodor Escherich isolates and describes Bacterium coli commune (later renamed Escherichia coli) from infant stool samples. This discovery marks…
Laboratory Strains of Escherichia coli
Laboratory strains of Escherichia coli represent specialized variants that have been developed and optimized for research and biotechnology applications. These…
Escherichia coli
Escherichia coli, commonly referred to as E. coli, is a Gram-negative, facultative anaerobic bacterium that belongs to the Enterobacteriaceae family. …
Growing Escherichia coli for Plasmid Isolation
Amplification of plasmid is desirable for many applications including gene cloning, DNA sequencing, transfection, and probe preparation. The fastest and routinely used method to amplify plasmid is to introduce plasmid in an appropriate strain of E. coli e.g., DH5α (the process is called transformation), grow them to a suitable culture volume, and finally, extract plasmid from them (the process called plasmid isolation).