Tag: plasmid
Plasmid pSRQ700
Naturally present in Lactococcus lactis subsp. cremoris DCH-4 (Moineau et al., 1995) Size: 7.8 kb (Moineau et al., 1995) Encodes…
Protocol: Size Determination of Plasmid DNA Using Agarose Gel Electrophoresis
Plasmids are circular DNA. Their size can not be determined in their native state as the migration of circular DNA in agarose gel is quite different and can not be compared with the linear fragments of ladder DNA. To know the approximate size of plasmid, the first step is to linearize the plasmid using a restriction enzyme that generates strictly a single cut. This results in a linear DNA that can be run on agarose gel and its size can be compared with the ladder sequence.
Principle of Alkaline Lysis Method
The alkaline lysis method selectively purifies plasmid DNA from other cellular components of the bacterial cells including chromosomal DNA. Controlled…
Plasmid Isolation by Alkaline Lysis Method
Alkaline lysis method of plasmid isolation was originally developed by Birnboim & Doly (1979). In this procedure, bacteria harbouring the desired…
Protocol – Growing Large Volume of Liquid Culture of Escherichia coli for Large-Scale Plasmid Isolation
Large-scale isolation of plasmid requires a large volume of E. coli culture. Large scale plasmid isolation procedures are termed, midiprep (25 – 50 ml starting culture volume) and maxiprep (100 – 500 ml starting culture volume). A starter culture is initially prepared by inoculating a colony in a small volume (2 – 10 ml) of culture medium. Large culture volume is prepared by diluting starter culture in a ratio of 1: 100 to 1: 1000 in the growth medium.
Protocol – Plasmid Isolation by Boiling Method (Miniprep)
Overview The boiling lysis method of plasmid isolation is quick and is recommended for the isolation of small plasmids (up…
Recommended Antibiotic Concentrations for the Selection and Growth of Escherichia coli Carrying a Plasmid
Most of the commonly used plasmid vectors carry at least one antibiotic resistance gene that allows plasmid-containing bacterial cells to…
Protocol – Preparing Liquid Culture of E. coli for Plasmid Miniprep
Overview:
Small-scale plasmid isolation, called plasmid miniprep, requires a small amount of bacterial culture. Normally, a 2 – 5 ml culture provides sufficient cells that can be processed for plasmid isolation using the miniprep protocol. Culture can be prepared by inoculating 3 ml antibiotic-containing growth medium with a single colony and growing it overnight at 37C with shaking.
Here we have taken an example of preparing liquid culture from a colony of E. coli DH5α, transformed with the pEGFP plasmid. The pEGFP plasmid contains a kanamycin resistance gene, therefore, requires kanamycin for the selection of plasmid-containing bacteria. If your plasmid carries another antibiotic-resistant gene, add the respective antibiotic to the culture medium.
Preparation of Glucose-containing Resuspension Buffer for Plasmid Isolation by Alkaline Lysis Method
Overview: Resuspension buffer is used to resuspend the harvested bacterial cells from the culture during plasmid isolation by the alkaline…
Preparation of Neutralization Solution (Solution III) for Plasmid Isolation by Alkaline Lysis Method
Overview The neutralization solution (solution III) is used for the isolation of plasmid DNA by the alkaline lysis method. The…
Preparation of Lysis Solution (Solution II) for Isolation of Plasmid by Alkaline Lysis Method
Overview Lysis solution (solution II) is used for the isolation of plasmid DNA by the alkaline lysis method. The plasmid-containing…
Plasmid Isolation
To isolate plasmid from the host bacteria, cells are first lysed that lead to the release of plasmid and in subsequent steps, the plasmid is purified from the lysate. Purification of plasmid from the lysed cells are mostly dependent on the type of lysis method used to release plasmid in solution. For example, alkaline lysis which completely disrupts the bacterial cells leading to the release of cell components including both plasmid DNA and genomic DNA in denatured state relies on selective renaturation of only plasmid DNA in a perfect manner at the purification step. On the other hand, boiling lysis selectively releases only plasmid DNA from the bacterial cells. The purified plasmid can be further purified by a number of methods to obtain high quality plasmids. These methods are centrifugation in gradients of CsCl – ethidium bromide (EtBr), selective precipitation in high salt SDS, extraction with Phenol-chloroform, and hydroxylapatite and ion-exchange chromatography.
Growing Escherichia coli for Plasmid Isolation
Amplification of plasmid is desirable for many applications including gene cloning, DNA sequencing, transfection, and probe preparation. The fastest and routinely used method to amplify plasmid is to introduce plasmid in an appropriate strain of E. coli e.g., DH5α (the process is called transformation), grow them to a suitable culture volume, and finally, extract plasmid from them (the process called plasmid isolation).
Preparation of Resuspension Buffer Containing Tris and EDTA for Isolation of Plasmid by Alkaline Lysis Method
Overview: The resuspension buffer is used to resuspend bacterial cells during plasmid isolation by the alkaline lysis method. It provides…
Basic Steps in Plasmid Isolation
Plasmid isolation is a common task in molecular biology laboratories. Tasks as simple as amplifying a plasmid vector to complex…