Category: Cell Culture

Protocol – Feeding Suspension Culture (Partial Replacement of Medium)

Replacement of culture medium from suspension culture is a bit tricky job as cells float in the medium. Ideally, cells are harvested from the medium by centrifugation and then resuspended in a fresh culture medium. However, in practice, a part of the culture medium is replaced with a fresh culture medium without harvesting the cells. This procedure is associated with the loss of cells.

Protocol – Feeding Adherent Culture

Feeding adherent culture involves replacing the old consumed medium with the fresh growth medium. The purpose is to supply fresh…

Preparation of Stock Solution of L-Glutamine (200 mM)

Overview L-Glutamine (C5H10N2O3; CAS number: 56-85-9) is an important component of the cell culture medium. It is an alternative energy…

Feeding Cells

Feeding is an essential step to maintain cells in a healthy state in culture. Feeding of a culture is a…

Protocol – Thawing and Revival of Cryopreserved Cells

This protocol describes the revival process of cryopreserved adherent cells. The revival process involves rapid thawing of cryopreserved cells followed by removal of freezing medium which contains cryoprotectant, in this case, DMSO. The freezing medium can be removed immediately just after thawing the cells by centrifugation. Alternatively, cells are allowed to adhere to the culture dish followed by washing and addition of fresh growth medium.

How To Choose Appropriate Cell Line For Research?

Description of basic criteria to select a cell line for your research project.

Protocol – Cryopreservation of Adherent Cells Growing in Serum-free Medium

Overview A serum-free freezing medium is used to preserve cell lines which are maintained in the serum-free growth medium. Serum-free…

Bacterial Contamination in Cell Culture

Bacterial Contamination in Cell Culture

Bacterial contamination is one of the most common cell culture contamination. Poor aseptic culture conditions, including handling, incubator and laminar…

Protocol – Subculturing Adherent Cells Growing in Serum-containing Growth Medium using Trypsin-EDTA

Overview The Trypsin-EDTA method, also referred to as trypsinization, is the most commonly used method for passaging/subculturing of adherent cells….

Protocol – Passaging/Subculturing Suspension Culture

Suspension culture is passaged by diluting the existing culture. Since cells float in the medium in suspension culture, they are not treated with a trypsin-EDTA solution. To subculture a suspension cell line, a small amount of cell suspension from the existing culture is transferred to a culture dish containing fresh growth medium.

Passaging/subculturing cells

Cell culture is not static. Cells acquire changes when maintained for a long time in culture (Masters & Stacey, 2007)….

Passaging/Subculturing Methods for Cells

Several methods have been developed for passaging cells. Each method has its own advantages and drawbacks. Always check the cell…

Cryopreservation of Cells

Cryopreservation is an efficient way to preserve cells at ultra-low temperatures (below -135°C) which stops all physiological processes and biological aging. It is a routinely used technique in all cell culture laboratories.

Cell culture overview

Cell Culture

Culturing cells from multicellular organisms especially from animals (mammals, amphibians, insects, birds etc) refers to cell culture or animal cell culture.

Preparation of Freezing Medium Containing DMSO and Serum

Overview The freezing medium is used to preserve cell lines at ultra-low temperatures. This method of preserving cell lines is…