Category: Lab Notes: Microscopy
Immunohistochemistry
Immunohistochemistry (IHC) is a widely used laboratory technique that combines histology, immunology, and chemistry to detect and visualize the presence…
Electron Microscopy
Electron microscopy (EM) is a powerful imaging technique that uses a beam of electrons instead of visible light to visualize…
Fluorophore
A fluorophore is a fluorescent chemical compound that can absorb light at a specific wavelength and subsequently emit light at…
4Pi microscope
The 4Pi microscope is an advanced fluorescence microscopy technique designed to dramatically improve axial (z-axis) resolution beyond the diffraction limit…
Abbe Diffraction Limit
The Abbe diffraction limit is a fundamental concept in optical microscopy that describes the smallest resolvable feature size achievable with…
Diffraction-Limited System
A diffraction-limited system refers to an optical system whose resolution is restricted solely by the fundamental effects of diffraction, rather…
STED Microscopy
Stimulated Emission Depletion (STED) microscopy is a super-resolution fluorescence imaging technique that overcomes the diffraction limit of conventional light microscopy. …
Incucyte
The Incucyte is a live-cell imaging and analysis system designed to enable continuous, non-invasive monitoring of cell cultures within a…
Holographic Microscopy
Holographic microscopy is an advanced imaging technique that combines the principles of holography with microscopy to obtain high-resolution, three-dimensional images…
Stochastic Optical Reconstruction Microscopy
Stochastic Optical Reconstruction Microscopy (STORM) is a single-molecule super-resolution imaging technique that enables visualization of biological structures at resolutions down…
Photoactivated Localization Microscopy (PALM)
Photoactivated Localization Microscopy (PALM) is a single-molecule super-resolution imaging technique that overcomes the diffraction limit of light microscopy, allowing researchers…
MINFLUX
MINFLUX (Minimal Photon Fluxes) is an advanced single-molecule localization microscopy technique that achieves nanometer-scale resolution with unprecedented efficiency in photon…
Scanning Tunneling Microscope
The Scanning Tunneling Microscope (STM) is a powerful analytical instrument that enables scientists to image and manipulate surfaces at the…
Tissue Clearing
Tissue clearing is a revolutionary technique in biological imaging that renders opaque tissues transparent, allowing researchers to visualize deep structures…
CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel)
CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel) is a groundbreaking tissue clearing method that transforms intact biological tissues…