Category: Lab Notes: Biotechniques
Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS)
Time-of-Flight Secondary Ion Mass Spectrometry (TOF-SIMS) is a highly sensitive surface analysis technique that provides detailed chemical and molecular information…
ATP Assay
An ATP assay is a biochemical method used to measure the amount of adenosine triphosphate (ATP) in biological samples. ATP…
Fluorophore
A fluorophore is a fluorescent chemical compound that can absorb light at a specific wavelength and subsequently emit light at…
4Pi microscope
The 4Pi microscope is an advanced fluorescence microscopy technique designed to dramatically improve axial (z-axis) resolution beyond the diffraction limit…
Abbe Diffraction Limit
The Abbe diffraction limit is a fundamental concept in optical microscopy that describes the smallest resolvable feature size achievable with…
Diffraction-Limited System
A diffraction-limited system refers to an optical system whose resolution is restricted solely by the fundamental effects of diffraction, rather…
STED Microscopy
Stimulated Emission Depletion (STED) microscopy is a super-resolution fluorescence imaging technique that overcomes the diffraction limit of conventional light microscopy. …
Thin-Layer Chromatography (TLC)
Thin-Layer Chromatography (TLC) is a widely used planar chromatographic technique for separating and identifying compounds within a mixture. It is…
High-Performance Thin-Layer Chromatography (HPTLC)
High-Performance Thin-Layer Chromatography (HPTLC) is an advanced form of conventional thin-layer chromatography (TLC), designed to enhance separation efficiency, reproducibility, and…
Gas Chromatography – Mass Spectrometry (GC-MS)
Gas Chromatography – Mass Spectrometry (GC-MS) is a powerful analytical technique that combines the separation capability of gas chromatography with…
Liquid Chromatography – Mass Spectrometry (LC-MS)
Liquid Chromatography – Mass Spectrometry (LC-MS) is a powerful analytical technique that combines the physical separation capabilities of liquid chromatography…
QuikChange Site-Directed Mutagenesis Kit (Agilent Technologies)
The QuikChange Site-Directed Mutagenesis Kit is a molecular biology tool designed for the rapid and efficient introduction of specific nucleotide…
Incucyte
The Incucyte is a live-cell imaging and analysis system designed to enable continuous, non-invasive monitoring of cell cultures within a…
Holographic Microscopy
Holographic microscopy is an advanced imaging technique that combines the principles of holography with microscopy to obtain high-resolution, three-dimensional images…
Applications of Immortalized Cell Lines
Immortalized cell lines have become indispensable tools in modern biology, biotechnology, and medicine because they can proliferate indefinitely under suitable…