Category: Lab Notes: Biotechniques
Crystallography
Crystallography is a fundamental scientific technique used to determine the three-dimensional arrangement of atoms in crystalline materials. This powerful method…
Spectroscope
A spectroscope is an optical instrument designed to analyze the properties of light by dispersing it into its constituent wavelengths,…
Spectroscopic Probes
Spectroscopic probes are specialized molecules or molecular groups that provide detailed information about chemical and physical properties of systems through…
Biuret Assay
The Biuret Assay, also known as Biuret Method, is a classical biochemical technique used for the quantification of proteins based…
Spectrophotometer
A spectrophotometer is a fundamental analytical instrument used in laboratories to measure the interaction of light with samples. This sophisticated…
Beer-Lambert Law
The Beer-Lambert law, also known as Beer’s law or the Beer-Lambert-Bouguer law, is a fundamental principle in spectroscopy that describes…
Tissue Clearing
Tissue clearing is a revolutionary technique in biological imaging that renders opaque tissues transparent, allowing researchers to visualize deep structures…
CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel)
CLARITY (Clear Lipid-exchanged Acrylamide-hybridized Rigid Imaging/Immunostaining/In situ hybridization-compatible Tissue-hYdrogel) is a groundbreaking tissue clearing method that transforms intact biological tissues…
CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis)
CUBIC (Clear, Unobstructed Brain/Body Imaging Cocktails and Computational analysis) is a comprehensive tissue clearing method that uses simple, hydrophilic chemicals…
DISCO (Tissue Clearing Method)
DISCO (3DISCO, iDISCO, uDISCO) represents a family of organic solvent-based tissue clearing methods that have revolutionized the way researchers visualize…
SCALE (Sorbitol/Calf serum/Alpha-lipoic acid/Ellipsenone)
SCALE (Sorbitol/Calf serum/Alpha-lipoic acid/Ellipsenone) is a hydrophilic tissue clearing method that achieves transparency through hyperhydration while preserving fluorescent proteins and…
SeeDB (See Deep Brain)
SeeDB (See Deep Brain) is an optical clearing method that utilizes simple sugar solutions to render biological tissues transparent while…
Serial Two-Photon Tomography
Serial Two-Photon Tomography (STPT) is an advanced imaging technique that combines the high-resolution, depthpenetra-ting capabilities of two-photon microscopy with automated…
Optical Projection Tomography
Optical Projection Tomography (OPT) is a powerful imaging technique used to acquire detailed three-dimensional (3D) representations of biological samples, particularly…
Swept Confocally-Aligned Planar Excitation (SCAPE) Microscopy
Swept Confocally-Aligned Planar Excitation (SCAPE) microscopy is an advanced light-sheet imaging technique designed to rapidly capture high-resolution, volumetric data from…