Category: Lab Notes: Cell Biology

COP1 Subcellular Localization in Mammalian Cells

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In mammalian cells, COP1 is a nucleocytoplasmic shuttling protein, but unlike in plants, its regulation is primarily linked to growth…

COP1 Subcellular Localization in Plant Cells

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In plants, COP1 exhibits a dynamic subcellular localization that is tightly controlled by light conditions, making it a central regulator…

COP1 Subcellular Localization

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COP1 is an evolutionarily conserved E3 ubiquitin ligase that plays a central role in light signaling in plants and in…

Post-Translational Modifications in Proteolysis

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Post-translational modifications (PTMs) in proteolysis represent a crucial layer of regulation in protein quality control and turnover, linking chemical modifications…

Ubiquitin–Proteasome System

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The ubiquitin–proteasome system (UPS) is the primary pathway for targeted protein degradation in eukaryotic cells, ensuring that damaged, misfolded, or…

Microtubule Organizing Center

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The Microtubule Organizing Center (MTOC) is a specialized cellular structure that serves as the primary site for microtubule nucleation and…

CRM1-Mediated Nuclear Export

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CRM1, also known as exportin 1 or XPO1, is a key nuclear export receptor that mediates the transport of a…

Cell Immortalization

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Cell immortalization is a biological process where normal cells, which typically have a limited lifespan, are transformed into cells that…

HeLa Cells and the Development of the Polio Vaccine

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HeLa cells, derived from the cervical cancer of Henrietta Lacks in 1951 without her consent, have played a pivotal role…

Cell Immortalization – Timeline of Key Events

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Cell immortalization is defined as the process by which cells acquire the capacity to proliferate indefinitely, overcoming the normal replicative…

Advantages and Limitations of Immortalized Cell Lines

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Immortalized cell lines are powerful tools for research due to their scalability, ease of use, and reproducibility, but their artificial…

Nucleolar Organizer Region

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The nucleolar organizer region (NOR) is a chromosomal locus that plays a central role in the formation of the nucleolus…

Post-Mitotic Nucleolar Reassembly at rDNA Clusters

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As cells complete mitosis and enter the early G1 phase, one of the first and most essential events in nuclear…

TRPP Channel

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The Transient Receptor Potential Polycystin (TRPP) subfamily is a group of ion channels within the broader TRP channel superfamily, best…

TRPML Channel 

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The Transient Receptor Potential Mucolipin (TRPML) subfamily is a distinct group within the TRP channel superfamily, primarily localized to intracellular…