Category: Lab Notes: Molecular Biology
Escherichia coli BL21(DE3) CodonPlus
Escherichia coli (E. coli) BL21(DE3) CodonPlus is a specialized derivative of the BL21(DE3) strain designed to improve the expression of…
Escherichia coli BL21(DE3)pLysS
Escherichia coli (E. coli) BL21(DE3)pLysS is a widely used strain for controlled recombinant protein expression, particularly when working with proteins…
Escherichia coli BL21
Escherichia coli (E. coli) BL21 is one of the most commonly used bacterial strains for recombinant protein expression. Derived from…
Escherichia coli Expression System
The Escherichia coli (E. coli) expression system is one of the most widely used platforms for recombinant protein production in…
Yeast Expression System
Yeast expression systems offer a versatile platform for recombinant protein production, combining many advantages of both prokaryotic and higher eukaryotic…
Prokaryotic Expression System
Prokaryotic expression systems are powerful tools used to produce recombinant proteins by utilizing the cellular machinery of bacteria. These systems…
Ectopic Expression Vs Heterologous Expression
Criteria Ectopic Expression Heterologous Expression Remarks Definition Expression of a gene in a different tissue, cell type, or subcellular location…
Heterologous Expression System
Heterologous expression systems are experimental platforms used to express genes or proteins in a host organism that is different from…
Heterologous Expression
Heterologous expression refers to the production of a gene product — typically a protein — in a host organism, cell…
Ectopic Expression
Ectopic expression refers to the abnormal or unintended activation of a gene in a cell type, tissue, or developmental stage…
Isogenic Mutant
Isogenic mutants are genetically engineered organisms or cell lines that are identical to their parental (wild-type) counterparts in every aspect…
Plasmid: Geometry and Conformation
Plasmid geometry and conformation represent fundamental aspects of plasmid structure that influence their biological function and behavior in both natural…
Plasmid DNA Isolation by the Alkaline Lysis Method Vs Boiling Lysis Method
Criteria Alkaline Lysis Method Boiling Lysis Method Remarks Principle Selective chemical denaturation using alkaline pH and detergent to separate plasmid…
Laboratory Strains of Escherichia coli
Laboratory strains of Escherichia coli represent specialized variants that have been developed and optimized for research and biotechnology applications. These…
Deoxyribonucleotide
Deoxyribonucleotides are the fundamental building blocks of DNA, consisting of a deoxyribose sugar, a phosphate group, and one of four…