Category: Lab Notes

CHES

IUPAC Name 2-(cyclohexylamino)ethanesulfonic acid Synonym 2-(Cyclohexylamino)ethanesulfonic acid, N-Cyclohexyltaurine 2-(Cyclohexylamino)ethanesulfonic acid; 2-(N-Cyclohexylamino)ethanesulfonic acid; Ethanesulfonic acid, 2-(cyclohexylamino)-2-[N-CYCLOHEXYLAMINO]ETHANE SULFONIC ACID Empirical formula (Hill…

HL-60/MX2

OVERVIEW   Organism Human (Homo sapiens), 36 years, White, Female Source/Tissue of origin Peripheral blood, Acute promyelocytic leukemia Morphology Lymphoblast-like…

Suppliers: Trypsin-EDTA solution

Note: Trypsin-EDTA solution can be purchased from the following suppliers. You must visit the supplier’s product page to order and…

Cell Line: P-815

OVERVIEW   Organism Murine (mouse) (Mus musculus), ​​DBA/2 Source/Tissue of origin Mouse mastocytoma tumor Morphology Single, round cells Cell type…

Tricine

IUPAC name 2-[[1,3-dihydroxy-2-(hydroxymethyl)propan-2-yl]amino]acetic acid Synonym N-Tris(hydroxymethyl)methylglycine; N-(Tri(hydroxymethyl)methyl)glycine; N-[Tris(hydroxymethyl)methyl]glycine Empirical formula (Hill system) C6H13NO5 Molar mass 179.17112 g/mol CAS number 5704-04-1…

TES

IUPAC name 3-morpholin-4-ylpropane-1-sulfonic acid Synonym 2-[(2-Hydroxy-1,1-bis(hydroxymethyl)ethyl)amino]ethanesulfonic acid, N-[Tris(hydroxymethyl)methyl]-2-aminoethanesulfonic acid, TES free acid Molecular formula C7H15NO4S Molar mass 229.25 g/mol CAS…

Trizma

IUPAC name 2-amino-2-(hydroxymethyl)propane-1,3-diol Synonym TROMETHAMINE, Trometamol, Tham, Tris, Tris buffer, Trisamine, Tris(Hydroxymethyl)aminomethane, Pehanorm, Talatrol Empirical formula (Hill system) C4H11NO3 Molar…

Ampicillin vs Carbenicillin

Properties Ampicillin Carbenicillin Remarks Semi-synthetic Yes Yes Both antibiotics are Semi-synthetic derivatives of penicillin Antibiotic class β-lactam β-lactam Both antibiotics…

List of Antibiotics

Amikacin  Ampicillin  Cefoxitin  Chloramphenicol  Ciprofloxacin  Clindamycin  Doxycycline  Erythromycin  Fusidic acid  Kanamycin  Lomefloxacin  Nalidixic acid  Nitrofurantoin Piperacillin  Spectinomycin  Spiramycin  Streptomycin  Sulfamethaxozole …

Antibiotic

Antibiotics refers to antimicrobial agents that either kill bacteria (bactericidal) or inhibit their growth (bacteriostatic).

Molarity of Pure Water

OVERVIEW Molarity is the number of moles present in a liter of solution. Suppose 10 moles of a solute present…

Protocol: Visualizing DNA in agarose gel using ethidium bromide – Precast method

Ethidium bromide is a DNA staining dye and can be used to visualize DNA embedded in agarose gel. Both precast…

Cell Line: FTC-236

OVERVIEW   Organism Human (Homo sapiens), 42 years old Male Source/Tissue of origin Thyroid, Neck lymph node metastasis, Follicular thyroid…

Composition and Ingredients of DNA Loading Dye

The composition and concentration of DNA loading dye can vary a lot. However, all DNA loading dyes contain two essential…

Preparation of 6X DNA Gel loading buffer (Bromophenol blue, Orange G, Xylene Cyanol FF, EDTA and Ficoll 400)

A 6x DNA Gel loading buffer containing Orange G, Bromophenol blue, Xylene cyanol FF, EDTA and Ficoll 400 is prepared by dissolving 25 mg bromophenol blue, 25 mg xylene cyanol FF, 40 mg Orange G, 1.5 g Ficoll 400 and 1.2 ml of 0.5 M EDTA in 10 mM Tris.Cl, pH 7.6 to a final volume of 10 ml. The final solution (6x DNA loading dye) will contain 0.25% (w/v) bromophenol blue, 0.25% (w/v) xylene cyanol FF, 15% (w/v) Ficoll 400, 60 mM EDTA and 10 mM Tris.