Category: Lab Notes

Cell Line : U3067MG

Overview   Organism Human (Homo sapiens), 67 years old, Female Source/Tissue Glioblastoma Morphology   Cell type Glioma stem cell /…

U3053MG

Overview   Organism Human (Homo sapiens), 64 years old, Male Source/Tissue Glioblastoma Morphology   Cell type Glioma stem cell /…

Cell Line : U3068MG

Overview   Organism Human (Homo sapiens), 69 years old, Male Source/Tissue Glioblastoma Morphology   Cell type Glioma stem cell /…

Nestin

Nestin is an intermediate filament protein Encoded by the NES gene (in human) Found in undifferentiated central nervous system cells…

Preparation of LB-Agar Medium and Plates

OVERVIEW LB medium can be solidified by adding agar. Solidified culture medium is very useful for the selection and identification…

Preparation of 6X DNA Loading Dye (Bromophenol blue and Sucrose)

Overview DNA sample is mixed with the DNA loading dye prior to loading into the wells of agarose gel. A…

Collagen Binding Integrins

The following integrins act as receptors of collagen. α1β1 (Major receptor) α2β1 α10β1 α11β1

Extracellular Matrix (ECM)

Extracellular matrix can be defined as a matrix associated with the extracellular part of cells (cell surface) in the tissue….

Preparation of 6X DNA Loading Dye (Bromophenol blue and Sucrose) Alternative Method

OVERVIEW This method uses 40% sucrose solution instead of sucrose powder. This method is convenient and easy, and does not…

Preparation of 6x DNA Loading Dye (Xylene Cyanol FF and Sucrose) Alternative Method

OVERVIEW This method uses 40% sucrose solution instead of sucrose powder. This method is convenient and easy, and does not…