Category: Lab Notes

Preparation of 6X DNA Loading Dye (Orange G and Sucrose)

Overview DNA loading dye containing only one tracking dye, Orange G, can be used to analyze large and moderate size…

Preparation of 6X DNA Loading Dye (Orange G and Sucrose) Alternative Method

OVERVIEW This method uses 40% sucrose solution instead of sucrose powder. This method is convenient and easy, and does not…

High-density Reagents for DNA Loading Dye

DNA loading dye contains a high density reagent. The high-density reagent is added to facilitate the sample to be placed…

World Health Organization (WHO) Classification of Central Nervous System Tumors

WHO has classified the central nervous tumors into distinct groups. The first classification was published in 1979. The classification has…

Preparation of 6X DNA Loading Lye (Xylene Cyanol FF and Glycerol)

Overview DNA loading dye containing only one tracking dye, xylene cyanol FF, can be used to analyze smaller DNA fragments…

Preparation of 6X DNA Loading Dye (Bromophenol Blue and Glycerol)

Overview DNA loading dye containing only one tracking dye Bromophenol blue (BPB) can be used to analyze larger DNA fragments…

Preparation of 6X DNA Loading Lye (Bromophenol Blue and Ficoll 400)

Overview DNA loading dye containing only one tracking dye bromophenol blue can be used to analyze larger DNA fragments by…

Preparation of 6X DNA Loading Dye (Orange G and Ficoll 400)

Overview DNA loading dye containing only one tracking dye, Orange G, can be used to analyze large and moderate size…

Integrins

Integrins along with the cadherins, immunoglobulin superfamily and selectins comprise the four major groups of cell adhesion molecules. They are…

Preparation of 6X DNA Loading Dye (Xylene Cyanol FF and Ficoll 400)

Overview DNA sample is mixed with DNA loading dye prior to loading into the wells of agarose gel.  DNA loading…

Regulation of Anchorage-Independent Growth by Cyclin-dependent Kinase Inhibitors (CKIs)

Cyclin-dependent kinase inhibitors (CKIs), such as p21WAF1, p27KIP1, p16INK4a, or p18INK4c, has been found upregulated in most cell types when…

Regulation of Anchorage-Independent Growth by p27

p27, a member of cyclin-dependent kinase inhibitors (CKIs), is up-regulated in the suspension culture of most cell lines including cancer…

Regulation of Anchorage-Independent Growth by Ras

Expression of dominant active Ras mutant, H-RasV12, has been reported to overcome the requirement of anchorage for a cell to…