Category: Lab Notes

This step aimed to distribute cell suspension into fresh culture dishes. Usually, when the purpose is to maintain a culture…

Suspension culture is passaged by diluting the existing culture. Since cells float in the medium in suspension culture, they are not treated with a trypsin-EDTA solution. To subculture a suspension cell line, a small amount of cell suspension from the existing culture is transferred to a culture dish containing fresh growth medium.

Enzymatic treatments (e.g., Trypsin, Collagenase, Dispase) Mechanical means (Scraping by rubber policeman, Dislodging semi-adherent cells by vigorous shaking) Treatment with…

Cryopreservation is an efficient way to preserve cells at ultra-low temperatures (below -135°C) which stops all physiological processes and biological aging. It is a routinely used technique in all cell culture laboratories.

Culturing cells from multicellular organisms especially from animals (mammals, amphibians, insects, birds etc) refers to cell culture or animal cell culture.

Further reading: Freshney, R. I., 2010. Basic Cell Culture Protocols / Culture of Animal Cells: A Manual of Basic Technique…