- Cell Line: CHP-212
- Synonyms:
- CHP212
- CHP 212
- CHP212
- Culture Type: Cell line
- Keywords: Human neuroblastoma cell line
- Description: Established from the brain of a caucasian male patient with neuroblastoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 1 year 8 months (cellosaurus)
- Ethnicity: Caucasian (cellosaurus)
- Gender: Male
- Tissue: Brain
- Disease:
- Neuroblastoma
- Molecular subtype: MYCN Amp (depmap)
- Molecular subtype: MYCN Amp (depmap)
- Neuroblastoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type:-
- Morphology: Neuroblast
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time:-
- Gene expression:-
- Sequence variations:
- NRAS (Q61K mutation, Oncogenic Gain-of-function)
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- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: 1:1 mixture of Eagle’s Minimum Essential Medium and F12 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time:
- Split ratio: 1:10
- Medium Renewal: Every 2 to 3 days
- Cryopreservation:
- Freezing medium: Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium: Complete growth medium + 5% DMSO
- Applications:
- Neuroscience
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- Images:-
- CHP-212 cell image (Source: depmap)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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