- Cell Line: 1008
- Synonyms:
- HCC-1008
- Hamon Cancer Center 1008
- Culture Type: Cell line
- Keywords: Human Lung Adenocarcinoma Cancer Cell Line
- Description: Established from lymph node metastatic tumor cells from a black, 67-year-old female patient with breast invasive ductal carcinoma TNM Stage IIA, grade 3.
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: Adult
- Ethnicity: Black
- Gender: Female
- Tissue (origin): Breast, Mammary gland
- Tissue (isolation): Lymph node metastatic tumor
- Disease:
- Breast Invasive Ductal Carcinoma TNM Stage IIA, grade 3
- Primary/Metastasis: Metastatic (Lymph node)
- Molecular subtype ERpos HER2pos (depmap)
- Breast Invasive Ductal Carcinoma TNM Stage IIA, grade 3
- Year of origin: 1994
- Organism:
- Cell line features:
- Morphology: Epithelial-like
- Cell Type: Epithelial cell
- Growth mode: Adherent
- Life span: Infinite (continuous culture)
- Karyotype: Multiploid
- Doubling time:-
- Clinically relevant genes:
- Gene expression:
- Positive for the expression of Epithelial glycoprotein 2 (EGP2) (epithelial cell-specific marker)
- Positive for the expression of Cytokeratin 19 (epithelial cell-specific marker)
- Sequence variations (gene mutations/deletions/fusions/translocations):-
- Morphology: Epithelial-like
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: DMEM:F12 Medium + 10% FBS + 0.005 mg/ml Insulin + 0.01 mg/ml Transferrin + 30 nM Sodium selenite (final conc.) + 10 nM Hydrocortisone (final conc.) + 10 nM beta-estradiol (final conc.) + extra 2mM L-glutamine (for final conc. of 4.5 mM) (ATCC)
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Cell dissociation from substratum
- Cell dissociation solution: Cell Dissociation Buffer (GIBCO #13150-016)
- Harvesting time:
- Split ratio: 1:2
- Medium Renewal: Every 3 to 4 days (depending on cell density)
- Subculturing method: Cell dissociation from substratum
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 7.5% DMSO (ATCC)
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:-
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability
- American Type Culture Collection (ATCC): Product link
- References
- https://www.atcc.org/products/crl-2320
- https://www.cellosaurus.org/CVCL_1244
- https://depmap.org/portal/cell_line/ACH-001514?tab=overview
- Gazdar et al., 1998. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PMID-9833771; Full Text: Wiley (Download PDF)
- Images
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- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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