- Cell Line: HCT-15
- Synonyms:
- HCT15
- HCT 15
- HCT.15
- Culture Type: Cell line
- Keywords: Human epithelial colorectal Adenocarcinoma cell line
- Description: Established from the primary tumor, taken from the large intestine of a White male with Dukes Type C colorectal Adenocarcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age:-
- Ethnicity: White
- Gender: Male
- Tissue: Large intestine, Colon
- Disease: Colorectal Adenocarcinoma
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: Epithelial
- Morphology: Epithelial-like
- Growth mode: Adherent, monolayer
- Life span: Infinite (continuous culture)
- Karyotype: Pseudodiploid
- Doubling time: 20-25 h
- Tumorigenicity: Tumorigenic in nude mice
- Gene expression:
- Positive for carcinoembryonic antigen (CEA)
- Positive for keratin
- Sequence variations:
- KRAS (G13D mutation, Oncogenic, Gain-of-function) (depmap)
- TP53 (S241F mutation, Likely Oncogenic, Likely Loss-of-function) (depmap)
- PIK3CA (E545K mutation, Oncogenic, Gain-of-function) (depmap)
- PIK3CA (D549N mutation, Oncogenic, Gain-of-function) (depmap)
- KDR (S925F mutation, Likely Oncogenic, Likely Gain-of-function) (depmap)
- IDH1 (G97D mutation, Likely Oncogenic, Switch-of-function) (depmap)
- ERBB3 (N126K mutation, Likely Oncogenic, Unknown) (depmap)
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- Cell Type: Epithelial
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Enzymatic dissociation
- Cell dissociation solution: 0.25% (w/v) Trypsin-0.53 mM EDTA solution
- Harvesting time: Split sub-confluent culture
- Split ratio: 1:5-1:10
- Medium Renewal: 2 to 3 times per week
- Cryopreservation:
- Applications:
- High-throughput screening
- Toxicology
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- UK Health Security Agency Culture Collections: Product link
- DSMZ-German Collection of Microorganisms and Cell Cultures: Product link
- References:
- Images:-
- HCT-15 cell line image (Source: UK Health Security Agency Culture Collections)
- HCT-15 cell line image (Source: depmap)
- HCT-15 cell line image (Source: DSMZ)
- HCT-15 cell line image (Source: DSMZ)
- HCT-15 cell line image (Source: DSMZ)
- HCT-15 cell line image (Source: ATCC)
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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