- Cell Line: NCI-BL2126
- Synonyms:
- NCI-BL2126
- BL2126
- Culture Type: Cell line
- Keywords: Human Epstein-Barr virus (EBV) transformed B lymphoblast cell line
- Description: Epstein-Barr virus (EBV) transformed B lymphoblast cell line established from peripheral blood of a Caucasian, 65-year-old, male donor
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 65 years
- Ethnicity: Caucasian
- Gender: Male
- Smoking habit:-
- Tissue (origin): peripheral blood
- Disease: Normal
- Year of origin:-
- Organism:
- Cell line features:
- Cell Type: B-cell
- Morphology: lymphoblast
- Growth mode: Suspension, multicellular aggregates
- Life span: Infinite (continuous culture)
- Karyotype:-
- Doubling time: ~66 hours
- Microsatellite instability:-
- Special features:-
- Product:-
- Soft agar colony formation assay:-
- Tumorigenicity:-
- Gene expression:
- Sequence variations:
- For information, please visit
- https://depmap.org/portal/cell_line/ACH-002371?tab=mutations
- For information, please visit
- Cell Type: B-cell
- Handling and maintenance:
- Biosafety level: BSL 2 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: RPMI-1640 Medium + 10% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Cell dissociation solution: NA
- Harvesting time:-
- Seeding density: 2 to 3 x 105 viable cells/mL
- Recommended split ratio:-
- Medium Renewal: Add fresh medium every 2 to 3 days
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 5% DMSO
- Complete growth medium + 5% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial: 1 ml
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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