- Cell Line: NCI-H128
- Synonyms:
- NCI-H128
- NCIH128
- H128
- H-128
- Culture Type: Cell line
- Keywords: Human Small Cell Lung Carcinoma Cell line
- Description: Established from the Pleural effusion of a 60-year-old, black, male patient with small cell lung carcinoma
- Origin:
- Organism:
- Species: Human (Homo sapiens)
- Age: 60 years
- Ethnicity: Black
- Gender: Male
- Tissue (origin): Lung
- Tissue (isolation): Pleural effusion
- Disease:
- Small cell lung carcinoma
- Primary/Metastasis: Metastatic
- Year of origin:
- Organism:
- Cell line features:
- Cell Type:
- Morphology: Floating aggregates
- Growth mode: Suspension
- Life span: Infinite (continuous culture)
- Karyotype: Aneuploid
Doubling time:- - Microsatellite instability: Stable
- Special features:
- Product:-
- Soft agar colony formation assay: form colonies in soft agar
- Tumorigenicity: Produce tumors in nude mice
- Gene expression:
- Antigen
- Negative for Dopa decarboxylase
- Negative for TAG-72 antigen
- Positive for CA19-9 antigen
- Antigen
- Sequence variations:
- Cell Type:
- Handling and maintenance:
- Biosafety level: BSL 1 (ATCC) (It may differ in your country, follow the guidelines of your institute/country)
- Complete culture medium: Iscove’s Modified Dulbecco’s Medium + 20% FBS
- Culture conditions:
- Temperature: Humidified 37°C incubator
- Atmosphere: 5% CO2, 95% Air
- Temperature: Humidified 37°C incubator
- Subculturing:
- Subculturing method: Dilution
- Harvesting time:-
- Seeding density:-
- Split ratio: 1:2 to 1:4
- Medium Renewal: Twice per week
- Subculturing method: Dilution
- Cryopreservation:
- Freezing medium:
- Complete growth medium + 10% DMSO
- Complete growth medium + 10% DMSO
- Storage conditions: Vapor phase of liquid nitrogen
- Cell suspension (cell density):-
- Volume per vial:
- Freezing medium:
- Applications:
- Cancer research
- 3D cell culture
- Availability:
- American Type Culture Collection (ATCC): Product link
- American Type Culture Collection (ATCC): Product link
- References
- Images:
- Abbreviations
- BSL (Biosafety level)
- FBS (Fetal Bovine Serum)
- DMSO (Dimethyl sulfoxide)
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